Phospho-YBX1 (S102) Antibody
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Description
Introduction
The Phospho-YBX1 (S102) Antibody is a polyclonal rabbit immunoglobulin (IgG) designed to detect phosphorylation at serine 102 (S102) of the Y-box binding protein 1 (YBX1), a multifunctional DNA/RNA-binding protein implicated in cancer progression, transcriptional regulation, and drug resistance . This antibody is widely used in research for studying YBX1 signaling pathways and its role in oncogenic processes.
Mechanism of YBX1 S102 Phosphorylation
Phosphorylation at S102 is mediated by the PI3K/AKT pathway and is critical for YBX1’s nuclear translocation and activation of oncogenic target genes . This modification facilitates YBX1’s role in promoting tumor growth, particularly in breast cancer, where it enhances cell proliferation and survival .
Research Findings on S102 Phosphorylation
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Breast Cancer: S102 phosphorylation correlates with nuclear YBX1 accumulation, which is associated with multidrug resistance and poor prognosis .
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Site-Specific Function: Mutation of S102 (S102A) prevents nuclear translocation, reducing tumor growth in MCF7 breast cancer cells .
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Pathway Crosstalk: S102 phosphorylation is induced by IGF-1 signaling, while IL-1β stimulation targets a distinct site (S165) in colon cancer models .
Applications in Research and Diagnostics
The antibody is primarily used in Western blot to detect S102-phosphorylated YBX1 in cell lysates or tumor tissues. For example, studies employing this antibody have demonstrated:
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Differential phosphorylation patterns between cancer cell lines (e.g., MCF7 vs. HEK293) .
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Validation of YBX1 as a therapeutic target in cancers with elevated S102 phosphorylation .
Clinical Relevance
YBX1 S102 phosphorylation is a biomarker for aggressive cancer phenotypes. Its inhibition has been proposed as a strategy to suppress oncogenic signaling pathways, including NF-κB activation .
Product Specs
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Lead Time
Typically, we can ship products within 1-3 business days of receiving your order. Delivery times may vary depending on the purchasing method or location. Please consult your local distributor for specific delivery timelines.
Synonyms
BP 8 antibody; CBF-A antibody; CCAAT binding transcription factor I subunit A antibody; CCAAT-binding transcription factor I subunit A antibody; CSDA2 antibody; CSDB antibody; DBPB antibody; DNA binding protein B antibody; DNA-binding protein B antibody; EFI-A antibody; Enhancer factor I subunit A antibody; MDR NF1 antibody; MGC104858 antibody; MGC110976 antibody; MGC117250 antibody; NSEP 1 antibody; NSEP1 antibody; Nuclease sensitive element binding protein 1 antibody; Nuclease-sensitive element-binding protein 1 antibody; p50 antibody; Q15905 antibody; Y-box binding protein 1 antibody; Y-box transcription factor antibody; Y-box-binding protein 1 antibody; YB 1 antibody; YB-1 antibody; YBOX1_HUMAN antibody; YBX 1 antibody; ybx1 antibody
Target Names
YBX1
Uniprot No.
Target Background
Function
YBX1, a DNA- and RNA-binding protein, plays a crucial role in a multitude of cellular processes including translational repression, RNA stabilization, mRNA splicing, DNA repair, and transcription regulation. Predominantly functioning as an RNA-binding protein, YBX1 exhibits a preference for the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C). It contributes to mRNA stabilization by binding to m5C-containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA degradation. As a component of the CRD-mediated complex, YBX1 promotes MYC mRNA stability. It further regulates translation by modulating the interaction between mRNA and eukaryotic initiation factors. YBX1 plays a pivotal role in the RNA composition of extracellular exosomes, defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs, and miRNAs. This sorting mechanism likely involves recognition and binding of C5-methylcytosine (m5C)-containing RNAs. As a key effector in epidermal progenitors, YBX1 prevents their senescence by regulating the translation of a senescence-associated subset of cytokine mRNAs, potentially through binding to m5C-containing mRNAs. YBX1 is also involved in pre-mRNA alternative splicing regulation, binding to splice sites in pre-mRNA and influencing splice site selection. Additionally, YBX1 demonstrates DNA-binding capabilities, regulating the transcription of the multidrug resistance gene MDR1. This regulation is enhanced in the presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. YBX1 binds to promoters containing a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Furthermore, YBX1 promotes the separation of DNA strands containing mismatches or modifications by cisplatin. Possessing endonucleolytic activity, YBX1 can introduce nicks or breaks into double-stranded DNA, suggesting a role in DNA repair. The secreted form of YBX1 functions as an extracellular mitogen, stimulating cell migration and proliferation.
Gene References Into Functions
- YBX1 is identified as a posttranscriptional effector essential for maintaining epidermal homeostasis. PMID: 29712925
- Phosphorylation of nuclear YB-1 following epidermal growth factor stimulation is linked to nuclear accumulation of p90 ribosomal S6 kinase but not YB-1. PMID: 30126195
- This study unveils a novel lncRNA, lncRNA-AWPPH, which is highly expressed in hepatocellular carcinoma (HCC). This lncRNA indicates a poor prognosis for HCC patients and promotes HCC cell proliferation, migration, and in vivo tumor growth and metastasis through a novel regulatory mechanism involving interaction with YBX1. PMID: 28428004
- YB-1 regulates miR-155 expression via c-Myb in laryngeal squamous cell carcinoma. Laryngeal squamous cell carcinoma patients with high YB-1 expression exhibit a poor overall survival rate. PMID: 29517281
- Infiltrating CD4 + T cells promote TGFbeta1 expression in both renal cell carcinoma and T cells, regulating renal cell carcinoma cell proliferation by modulating TGFbeta1/YBX1/ HIF2alpha signals. PMID: 29289594
- This study elucidates the structural basis of interactions between YB-1 and mRNAs containing the aforementioned motifs. PMID: 29133115
- Silencing of YB-1 sensitizes SH-SY5Y cells to cisplatin and promotes cisplatin-induced apoptosis through down-regulation of multidrug resistance (MDR) 1 protein via the NF-kappaB signaling pathway. PMID: 28382490
- These findings suggest that extracellular vesicles and exosomes might play a role in purging and intercellular transfer of excess free RNAs, including full-length transfer RNAs and other small noncoding RNAs. PMID: 29073095
- YB-1 promotes lung adenocarcinoma growth and progression in vitro and in vivo by directly binding to the MACC1 promoter and enhancing the MACC1/c-Met pathway. PMID: 28624808
- Data indicate that YB-1 translocates to the nucleus concurrently with p53 expression and is involved in gemcitabine resistance in bladder cancer, suggesting that nuclear expression of YB-1 is crucial for resistance to chemotherapy, including gemcitabine, in TP53-mutated bladder cancer. PMID: 28714514
- Results demonstrate that TP53TG1 regulates YBX1 expression by binding to the YBX1 promoter and preventing its nuclear localization. PMID: 27821766
- Kindlin-2 is up-regulated in glioma cells and acts as an oncogene, serving as an independent risk factor for poor prognosis. The Kindlin-2/YB-1/beta-catenin complex promotes EGFR transcription and contributes to glioma progression. Kindlin-2 is a potential diagnostic and prognostic marker in glioma, and inhibiting Kindlin-2 could be a novel therapeutic strategy for glioma treatment. PMID: 27713156
- Findings indicate that YB-1 promotes hepatocellular carcinoma (HCC) cell proliferation, migration, and colony formation. Moreover, YB-1 is found to increase drug resistance in HCC. These findings suggest that YB-1 is a key factor in HCC tumorigenesis and maintains the HCC initiating cell population. PMID: 27911878
- Furthermore, combining indirubin 3'-oxime and actinomycin D enhances the anticancer effect on HepG2 cells. Indirubin 3'-oxime is a novel and effective inhibitor of anticancer agent-induced YB-1 nuclear translocation. PMID: 29274785
- It appears that YB-1 could regulate cell invasion and migration by downregulating its indirect target, coronin-1C. The association between YB-1 and coronin-1C offers a novel approach to target and potentially abrogate breast cancer cell metastasis. PMID: 28302118
- Inhibition of YBX1 suppresses lung cancer growth partly through the CDC25a pathway, and high expression of YBX1/CDC25a predicts a poor prognosis in human lung adenocarcinoma. PMID: 27384875
- The long noncoding RNA CAR intergenic 10 binds and stabilizes transcription factor Y-box-binding protein 1 (YB-1), leading to up-regulation of the epidermal growth factor receptor (EGFR) and proliferation of lung cancer cells. PMID: 27322209
- Results initially reported that YB-1, whose mRNA expression is regulated by HPV18 E6, promotes epithelial-mesenchymal transition and progression of cervical cancer by enhancing the expression of Snail. PMID: 28708785
- YB-1 localization is significantly altered in the nuclei of tumor blasts derived from bone marrow or peripheral blood. PMID: 28009354
- Two interacting partners of urokinase-type plasminogen activator (uPA) receptor, the cysteine-rich angiogenic inducer 61 (Cyr61) and the Y-box-binding protein 1 (YB-1) are identified and their differential expression is demonstrated in triple-negative breast cancer (TNBC) cells and tumors. PMID: 27286449
- No other significant associations are found between clinical prognostic factors and YB-1/p18 serum levels. PMID: 27371774
- Data suggest that oncogenic Y-box binding protein 1 (YB-1) indirectly enhances transforming growth factor beta (TGFbeta) signaling cascades through Sma/Mad related protein 2 (Smad2) phospho-activation and might represent a promising factor for future diagnosis and therapy of breast cancer. PMID: 29187452
- High YBX1 expression is associated with invasion in nasopharyngeal carcinoma. PMID: 29024700
- TCTP interacts with Y-box-binding protein 1 (YBX1), and their interaction is localized to the N-terminal region of TCTP and the 1-129 amino acid (aa) residues of YBX1. The interaction of TCTP with YBX1 might cooperate or coordinate their functions in the control of diverse regulatory pathways in cancer cells. PMID: 27607350
- YB-1 expression promotes pancreatic cancer metastasis, which is inhibited by microRNA-216a. PMID: 28782557
- Findings point to YB-1 and NSUN2 as possible mediators of the process of transferring specific mRNAs into exosomes, allowing us to speculate on an involvement of these proteins in mRNA sorting through the recognition of the aforementioned motifs. PMID: 28341602
- YB-1 is induced by Shh in CGNPs. PMID: 26725322
- FOXO3a nuclear expression is inversely correlated with YB-1 nuclear expression in prostate cancer tissues. PMID: 27699813
- These findings define a mechanism of drug resistance through which YBX1 contributes to antiestrogen bypass in breast cancer cells. PMID: 27879270
- High YB1 expression is associated with Cisplatin Resistance in Gastric Cancer. PMID: 27013200
- Collectively, these findings demonstrate a novel role of YBX1 in maintaining the stemness of CSCs and metastasis, unveiling YBX1 as a promising therapeutic target for NSCLC treatments. PMID: 28400280
- These studies reveal the functional role of YB-1 in the Angiogenin-mediated stress response program. PMID: 27174937
- Together, these results indicate that transportin-1 mediates YB-1 nuclear translocation. PMID: 27794479
- YB-1 is a novel binding factor for hypoxia-responsive elements that participates in the fine-tuning of the hypoxia transcriptome. PMID: 27524241
- The long noncoding RNA highly up-regulated in liver cancer (HULC) promotes the phosphorylation of YB-1 through the extracellular signal-regulated kinase pathway, in turn regulates the interaction of YB-1 with certain oncogenic mRNAs, and consequently accelerates the translation of these mRNAs in the process of tumorigenesis. PMID: 28027578
- Glycosylated-YB-1 primarily enhances cell proliferation through synergistic actions with YB-1 phosphorylation, playing indispensable roles in fine-tuning cell proliferation. PMID: 27751836
- Silencing YB-1 sensitizes DLBCL cells to mitoxantrone and overcomes the cell adhesion-mediated drug resistance (CAM-DR) phenotype in an AKT-dependent manner. PMID: 27397581
- These data suggest that C1QBP could regulate YBX1 to suppress AR-enhanced RCC cell invasion. Targeting this newly identified C1QBP/YBX1/AR/MMP9 signaling pathway may provide a new potential therapeutic strategy to better suppress RCC metastasis. PMID: 28107702
- Tumor growth and liver metastasis formation are significantly suppressed in nude mice after implantation of YB-1-silenced PDAC cells, and the YB-1 targeting antisense oligonucleotide significantly inhibits the growth of subcutaneous tumors. PMID: 26939718
- We propose a model whereby high YB-1 levels in certain TNBC cells can lead to reduced levels of EGR1, which in turn promotes slow cell cycling and resistance to PTX. Therefore, YB-1 and EGR1 levels are biologically linked and may provide a biomarker for TNBC response to PTX. PMID: 27072400
- LL37 induces YB1 expression, and increased tumor cell proliferation, migration, and invasion of A375 and A875 malignant melanoma cell lines. PMID: 27922666
- Down-regulation of miR-137 confers OXA resistance in parental cells, while over-expression of miR-137 sensitizes resistant cells to OXA, which is partly rescued by YBX1 siRNA. PMID: 28035913
- This finding suggests that selective inhibition of translation of YB-1 mRNA, and probably some other mRNAs as well, by mTOR kinase inhibitors is not mediated by the action of the 4E-binding protein upon functions of the 4F-group translation initiation factors. PMID: 26931209
- Cytoplasmic expression of YB-1 is associated with more aggressive tumor and poor overall survival in breast cancer. PMID: 26193840
- High-grade nuclear YB-1 expression is detected in 62.9% of colorectal cancer cases and is found to be an independent predictor of poorer overall survival and relapse-free survival. PMID: 27354655
- This study demonstrates that DeltaNp63alpha controls YB-1 protein stability, suggesting that DeltaNp63alpha/YB-1 crosstalk is relevant for the survival of basal keratinocytes in stratified epithelia. PMID: 27168020
- These findings demonstrate the oncogenic roles of YB-1 in esophageal squamous cell carcinoma (ESCC) and support it as a target for ESCC therapy. PMID: 27044807
- Immunofluorescence staining is used to detect YB-1 levels in the cytoplasm and nucleus. Results indicate that intracellular distribution is significantly associated with the pathological grade of glioma. PMID: 26936129
- Results show that high GOLPH3 and nuclear/cytoplasmic YB-1 expression correlate with a poor prognosis in patients with prostate cancer. PMID: 26794392
- Data suggest that poly(ADP-ribosyl)ation of YB-1 (multifunctional Y-box binding protein 1) is catalyzed by PARP1 [poly(ADP-ribose)polymerase 1] and can be up-regulated by damaged DNA. PMID: 26453809
Database Links
Subcellular Location
Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Secreted, extracellular exosome.
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