PHYHIPL Antibody
Description
Research Applications
PHYHIPL antibodies are validated for multiple techniques:
| Application | Recommended Dilution | Sample Types |
|---|---|---|
| Western Blot (WB) | 1:100–1:5000 | Brain/testis lysates (mouse, rat, human) |
| Immunofluorescence (IF) | 1:50–1:500 | Fixed mouse brain tissue |
| Immunohistochemistry (IHC) | 1:50–1:500 | Paraffin-embedded brain/testis tissue |
| ELISA | 1:30–1:3000 | Solid-phase assays |
Optimization Notes:
Biological Significance of PHYHIPL
PHYHIPL is implicated in critical cellular processes:
Mitochondrial Dynamics:
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Associated with energy production, oxidative stress regulation, and organelle dynamics .
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Interacts with APOE, a protein linked to mitochondrial dysfunction during ischemia .
Disease Associations:
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Cancer: Downregulated by IRX1, a tumor suppressor gene in gastric carcinoma .
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Neurodevelopment: Participates in central nervous system development .
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Ischemic Injury: Protein levels fluctuate during global ischemia, influenced by APOE genotypes .
Technical Considerations
Validation Data:
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Western Blot: Clear bands at 39 kDa and 42–45 kDa in brain/testis lysates .
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Immunoprecipitation: Effective at 1–2 µg antibody per 100–500 µg lysate .
Research Findings
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Isoform Diversity: Three splice variants (6 kDa, 40 kDa, 42 kDa) are differentially expressed across tissues .
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Conservation: PHYHIPL is evolutionarily conserved in vertebrates, including zebrafish and chickens .
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Pathological Relevance: Altered PHYHIPL expression correlates with mitochondrial dysfunction in ischemic and cancerous states .
