PIP5K1 Antibody

Definition and Biological Significance

PIP5K1 (Phosphatidylinositol-4-phosphate 5-kinase type I) is an enzyme critical for synthesizing phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂), a lipid signaling molecule involved in membrane trafficking, cytoskeletal remodeling, and viral replication . The PIP5K1 family includes three isoforms (α, β, γ), with PIP5K1α being the most abundant and functionally distinct . PIP5K1 antibodies are designed to detect PIP5K1α protein, enabling studies of its subcellular localization, enzymatic activity, and role in disease pathologies like cancer and viral infections.

3.1. Western Blot (WB)

• Detection: PIP5K1α protein levels in lysates from cancer cells (e.g., C4-2, DU145) or viral-infected cells (e.g., TZM-bl HeLa) .

• Dilution: 1:250–1:50,000 (optimized per antibody) .

3.2. Immunohistochemistry (IHC)

• Tissue: Human tonsil, liver, prostate tumors .

• Antigen Retrieval: TE buffer (pH 9.0) or citrate buffer (pH 6.0) .

3.3. Immunofluorescence (IF)

• Localization: Golgi apparatus, endomembranes, or plasma membrane (PM) in HIV-1-infected cells .

3.4. Immunoprecipitation (IP)

• Interactions: PI3K/AKT pathway components in cancer models .

4.1. HIV-1 Replication

• Role of PIP5K1α: Required for PI(4,5)P₂ production at the PM, enabling HIV-1 Gag (Pr55) targeting to viral assembly sites .

• Depletion Effects:

  • PIP5K1α Knockdown: Gag degradation via proteasome/lysosomes, reduced extracellular release .

  • PIP5K1γ Knockdown: Gag accumulation in late endosomes .

Data from .

4.2. Cancer Progression

• Castration-Resistant Prostate Cancer (CRPC): PIP5K1α promotes tumor growth, invasion, and PI3K/AKT signaling .

• Mechanisms:

  • siRNA Knockdown: ↓ AKT phosphorylation, ↓ cyclin D1/A1, ↑ p27 (cell cycle arrest) .

  • ISA-2011B Inhibitor: Suppressed spheroid formation in C4-2 and DU145 cells .

4.3. PI(4,5)P₂ Metabolism

• Dominance of PIP5K1α: Accounts for 70–80% of total PI(4,5)P₂ in HeLa cells; silencing reduces PI(4,5)P₂ levels by 80% .