BHLH72 Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- BHLH72 regulates the expression of genes responsive to shade conditions. It recruits MRG1 and MRG2, which bind to H3K4me3/H3K36me3 and attract histone-acetylases to induce histone acetylation, promoting the expression of shade-responsive genes. PMID: 29187567
- Newly synthesized auxin, generated through a BHLH72-regulated pathway, is essential for shade-induced growth, directly connecting the perception of light quality signals to a rapid growth response. PMID: 22508725
- Research indicates that BHLH72 functions in the phyB signaling pathway under prolonged red light by maintaining low phyB protein levels, potentially in an additive or synergistic manner. PMID: 18252845
Q&A
The BHLH72 antibody represents an emerging tool in antibody engineering, with applications in both basic research and therapeutic development. Below are structured FAQs addressing key scientific considerations, derived from current methodologies in antibody discovery and specificity engineering.
Advanced Research Questions
How can computational models resolve contradictions in BHLH72’s apparent cross-reactivity?
Discrepancies arise when ligands share overlapping epitopes. Solutions include:
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Biophysics-informed energy modeling to disentangle binding modes for chemically similar ligands .
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Network integration of transcriptomic data to identify pathways influencing off-target interactions (e.g., BCR signaling) .
Example Workflow
What strategies optimize BHLH72’s affinity without compromising specificity?
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Directed evolution: Iterative rounds of error-prone PCR coupled with yeast display screening .
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Paratope engineering: Computational redesign of CDR loops using RosettaAntibody .
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In vivo maturation: Transgenic mouse models expressing human Ig loci to refine affinity .
Methodological Challenges
How to address low-titer BHLH72 production in recombinant systems?
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Codon optimization: Adjust coding sequences for improved expression in mammalian (e.g., HEK293) or microbial systems.
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Chaperone co-expression: Co-express folding chaperones like Bip to reduce misfolding .
What controls are essential when interpreting BHLH72’s binding in immunohistochemistry?
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Isotype-matched controls to rule out Fc-mediated binding.
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Cell-line panels expressing varying levels of the target antigen .
Data Interpretation
How to distinguish genuine binding signals from assay artifacts in BHLH72 studies?
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Dual-labeling experiments: Combine BHLH72 with a second antibody targeting a non-overlapping epitope.
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Kinetic analysis: Surface plasmon resonance (SPR) to quantify on/off rates and identify non-specific avidity effects .
Key Validation Criteria
| Parameter | Acceptable Range |
|---|---|
| KD (SPR) | ≤10 nM |
| Cross-reactivity (ELISA) | ≤5% vs. homologs |
| Intra-assay CV | <15% |
