PKIA Antibody
Description
Definition and Target Specificity
PKIA Antibody detects endogenous PKIA, a 76-amino acid protein (8 kDa) that inhibits cAMP-dependent protein kinase A by binding to its catalytic subunits . This antibody is crucial for studying PKIA's role in cellular processes like calcium regulation and mitochondrial dynamics .
| Key Target Attributes | Details |
|---|---|
| Gene Symbol | PKIA |
| UniProt ID | P61925 |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Synthesized peptide (AA 10-59 in humans) or recombinant fusion proteins |
Research Applications
PKIA antibodies are validated for multiple techniques, with dilution protocols optimized per application:
Research Findings
Recent studies utilizing PKIA antibodies highlight its regulatory roles:
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PKA Inhibition: PKIA suppresses PKA activity by sequestering catalytic subunits, impacting phosphorylation of CREB and β-catenin .
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Mitochondrial Dynamics: Reduced PKIA expression increases ER calcium stores via DRP1 phosphorylation, influencing mitochondrial fission .
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Disease Associations: Elevated PKIA levels correlate with cancer progression and metabolic disorders in murine models .
Product Specs
Target Background
This antibody serves as an exceptionally potent competitive inhibitor of cAMP-dependent protein kinase activity. It interacts directly with the catalytic subunit of the enzyme following the cAMP-induced dissociation of its regulatory chains.
