PKIG Antibody
Description
Introduction
The PKIG antibody (Catalog No. 21371-1-AP) is a rabbit-derived polyclonal immunoglobulin G (IgG) designed to target Protein Kinase (cAMP-dependent, catalytic) Inhibitor gamma (PKIG). This antibody is widely used in molecular biology research, including Western blot (WB), immunofluorescence (IF/ICC), and enzyme-linked immunosorbent assay (ELISA) applications. Its specificity for PKIG, a critical regulator of cAMP-dependent protein kinase activity, makes it a valuable tool for studying cellular signaling pathways .
Structure and Function
The PKIG antibody adheres to the canonical Y-shaped structure of immunoglobulins, comprising two heavy chains and two light chains (κ or λ isotype). The heavy chains determine the antibody’s class (IgG, IgA, etc.), while the light chains contribute to antigen-binding diversity . The antibody’s functional regions include:
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Fragment Antigen-Binding (Fab): Binds specifically to PKIG via its variable domains (VH and VL).
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Fragment Crystallizable (Fc): Interacts with effector molecules, such as Fc receptors, enabling immune responses .
| Characteristic | Detail |
|---|---|
| Immunogen | PKIG fusion protein (Ag15878) |
| Host/Isotype | Rabbit / IgG |
| Molecular Weight | 76 aa (8 kDa observed) |
| Reactivity | Human, mouse, rat |
Applications and Reactivity
The PKIG antibody is validated for:
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WB: Detects PKIG in mouse heart tissue lysates (1:200–1:1000 dilution) .
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IF/ICC: Visualizes PKIG localization in HeLa cells (1:10–1:100 dilution) .
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ELISA: Quantifies PKIG levels in biological samples.
Its reactivity is species-specific, with no cross-reactivity reported for non-target proteins .
Pharmacokinetics and Efficacy
In vivo studies using murine models demonstrate dose-dependent clearance of the PKIG antibody:
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High-dose (100 mg/kg): Half-life ≈ 11 hours, with target-mediated elimination saturating at plasma concentrations >100 μg/mL .
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**Low-dose (1 mg/kg):**Accelerated clearance due to non-saturated target binding .
| Dose (mg/kg) | Half-life (hours) | Clearance (mL/h/kg) |
|---|---|---|
| 1 | 5.2 | 123.8 |
| 100 | 11.0 | 57.6 |
Published Research
The antibody has been cited in studies investigating PKIG’s role in:
Product Specs
Target Background
- Studies have shown that endogenous levels of Pkig reciprocally regulate osteoblast and adipocyte differentiation. This reciprocal regulation is partially mediated by LIF. PMID: 23963683
- These findings suggest that downregulation of PKIgamma may be a prerequisite for PKA activation during osteoblastic differentiation of precursor cells. PMID: 16870489
- Functional studies have been conducted on the mouse homolog. PMID: 9218452
