At4g00040 Antibody
Description
Research Applications of the At4g00040 Antibody
The antibody is primarily used to investigate At4g00040’s spatial-temporal expression and its role in plant biology. Key findings include:
Expression Profiling
Quantitative analysis in ams (ABORTED MICROSPORES) mutants shows reduced At4g00040 transcript levels during early anther development :
| Developmental Stage | Fold Change in ams Mutants |
|---|---|
| Stage 6 | -0.45 |
| Stage 8 | -1.30 |
| Stage 9–10 | No significant change |
These data suggest partial regulation by the AMS transcription factor, though the protein’s functional role remains unclear .
Functional Redundancy Testing
Despite structural similarity to LAP5/LAP6, transgenic overexpression of At4g00040 under LAP5 or LAP6 promoters failed to rescue pollen adhesion defects in lap5/lap6 mutants . This highlights its non-redundant role in pollen wall formation.
Technical Validation
The antibody’s utility is confirmed through:
-
Immunolocalization: Detects protein in vegetative tissues and early-stage anthers, consistent with its broad expression profile .
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Western Blotting: Identifies a ~42 kDa protein, aligning with predicted molecular weight .
Comparative Analysis with Related Proteins
At4g00040 differs functionally from other CHS-like proteins involved in sporopollenin biosynthesis:
| Protein | Expression Specificity | Role in Pollen Development | Mutant Phenotype |
|---|---|---|---|
| LAP5/LAP6 | Anther-specific | Sporopollenin synthesis | Pollen adhesion defects |
| At4g00040 | Broad | Undetermined | No observable defects |
Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
