PLA2G4E Antibody, FITC conjugated

General Characteristics

Species Reactivity

Biological Role of PLA2G4E

PLA2G4E (Group IVE phospholipase A2) is a calcium-dependent enzyme involved in lipid metabolism, particularly in synthesizing N-acyl ethanolamines (NAEs) like anandamide. Its activity impacts inflammation, cell signaling, and lysosomal membrane integrity .

Key Functional Insights

• Lysosomal Membrane Permeabilization (LMP):
  PLA2G4E inhibition via Mir504-5p or shRNA reduces LMP-induced necroptosis, enhancing survival of ischemic skin flaps in murine models .

• Enzymatic Mechanism:
  Transfers acyl chains from phosphatidylcholine to phosphatidylethanolamine, generating precursors for bioactive lipids .

Validation Data

Therapeutic Potential

• Ischemic Tissue Survival:
  PLA2G4E knockdown via AAV-shRNA improved random-pattern skin flap survival by 37% in mice by mitigating LMP and necroptosis .

• Neurological Studies:
  Linked to NAE biosynthesis in the brain, affecting endocannabinoid signaling pathways .

Technical Advantages

• High Specificity: Detects endogenous PLA2G4E without cross-reactivity to related phospholipases .

• Broad Compatibility: Validated in FFPE tissues, frozen sections, and cell cultures .

Recommended Workflow

1. Storage: Maintain at -20°C; avoid freeze-thaw cycles .

2. Dilution: Optimize for each assay (e.g., 1:50–1:100 for IHC, 1:40,000 for ELISA) .

3. Controls: Use A431 (positive) and Pla2g4e knockdown models (negative) .

Troubleshooting