acl-12 Antibody

Target Protein: TMEM16B/ANO2

Anoctamin-2 (ANO2/TMEM16B) is a calcium-activated chloride channel (CaCC) belonging to the TMEM16 protein family . Key features include:

• Structure: 10 transmembrane domains with intracellular N- and C-termini .

• Function: Mediates Ca²⁺-activated Cl⁻ currents in olfactory epithelium, photoreceptor synapses, and retinal pigment epithelium .

• Pathological Relevance: Linked to olfactory signal transduction, neuronal excitability, and retinal degeneration .

3.1. Western Blot Analysis

• Detects ANO2 in mouse brain lysate at ~110 kDa .

• Example: Zhang et al. (2015) confirmed ANO2 expression in mouse brain using ACL-012 .

3.2. Immunohistochemistry

• Localizes ANO2 in mouse olfactory bulb axons (Fig. 1A) .

• Colocalizes with synaptophysin in olfactory glomeruli (Fig. 1B–C) .

3.3. Functional Studies

• Role in Olfaction: Mura et al. (2017) used ACL-012 to demonstrate ANO2 expression in rat olfactory sensory neurons .

• Retinal Function: Keckeis et al. (2017) identified ANO2 in mouse retinal pigment epithelium using this antibody .

Key Research Findings

Technical Considerations

• Specificity: ACL-012 shows no cross-reactivity with other TMEM16 family members (e.g., TMEM16A) .

• Limitations: Requires validation via peptide preabsorption to confirm signal specificity .

Comparative Data

Expression Levels in Mouse Tissues (Western Blot) :

Clinical and Industrial Relevance

• Therapeutic Potential: ANO2 modulation is explored for treating olfactory dysfunction and retinal disorders .

• Biomarker Utility: Unlike TMEM16A (a cancer biomarker), ANO2 is primarily studied in neurological contexts .