PLC4 Antibody

Introduction

PLCβ4 and PLCD4 are distinct isoforms within the PLC family, each with unique biological functions. PLCβ4 is predominantly expressed in neuronal tissues and implicated in neurotransmitter signaling, while PLCD4 is associated with cancer progression. Antibodies against these proteins enable researchers to investigate their expression, localization, and mechanistic roles.

PLCβ4 Antibodies

PLCβ4 (Phospholipase C beta 4) regulates metabotropic glutamate receptor signaling in the cerebellum. Commercial antibodies targeting PLCβ4 include:

Table 1: Key PLCβ4 Antibodies

• Functional Insights:

  • PLCβ4 knockout mice exhibit cerebellar ataxia due to impaired climbing fiber synapse elimination .

  • Activating mutations (e.g., D630Y) in PLCβ4 are linked to uveal melanoma, promoting uncontrolled melanocyte proliferation .

PLCD4 Antibodies

PLCD4 (Phospholipase C delta 4) is implicated in cancer and immune regulation. Notable antibodies include:

Table 2: Key PLCD4 Antibodies

• Research Findings:

  • PLCD4 expression correlates with tumor progression in pancreatic and other cancers, serving as a potential biomarker .

  • It hydrolyzes phosphatidylinositol phosphates, influencing calcium signaling and cell migration .

Research Findings on PLCβ4 and PLCD4

• PLCβ4 in Neurological Disorders:

  • Cerebellar dysfunction in PLCβ4-deficient mice highlights its role in motor coordination .

  • Altered PLCβ4 activity disrupts Gαq-mediated signaling, contributing to developmental abnormalities .

• PLCD4 in Oncology:

  • Overexpression in cancer cells enhances invasiveness by modulating Wnt/β-catenin pathways .

  • Antibodies like 10589-2-AP enable detection of PLCD4 in human pancreatic cancer tissues .

Applications in Research and Diagnostics

• Western Blotting:

  • Monoclonal antibodies (e.g., ABIN968583) show high specificity for PLCβ4 in brain lysates .

• Immunohistochemistry:

  • PLCD4 antibodies localize the protein in tumor microenvironments, aiding cancer diagnostics .

• Functional Studies:

  • Knockout cell lines (e.g., THP-1 PLCG2 KO) validate antibody specificity in signaling studies .

Table 3: Performance Metrics

• Critical Considerations:

  • Recombinant monoclonal antibodies (e.g., Abcam’s EPR1403) reduce off-target binding in complex samples .

  • Polyclonal antibodies (e.g., HPA007951) offer broad epitope recognition but may require titration .