Customize plc-3 Antibody

Description

Introduction to PLC-β3 Antibody

PLC-β3 antibodies are immunoglobulin-based reagents designed to bind specifically to the Phospholipase C beta 3 enzyme (UniProt ID: Q01970), which catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP₂) into second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP₃) . These antibodies are essential for detecting PLC-β3 expression in human, mouse, and rat samples across techniques like Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) .

Research Applications

Western Blot Validation: Detects PLC-β3 at ~139 kDa (human) and ~150 kDa (murine) .
Functional Studies: Used to explore PLC-β3's role in immune cell activation, neutrophil differentiation, and cancer progression .
Post-Translational Modification Analysis: Phospho-specific antibodies (e.g., anti-Ser537) identify activated PLC-β3 in signaling pathways .

Role in Immune Regulation

PLC-β3 regulates Stat5 activity in hematopoietic stem cells (HSCs) by forming a multi-molecular complex (SPS complex) with the phosphatase SHP-1. This interaction inhibits Stat5 phosphorylation, thereby suppressing myeloproliferative neoplasms (MPNs) and aberrant neutrophil production .

Cancer and Apoptosis

In macrophages, PLC-β3 deficiency increases sensitivity to apoptosis due to reduced Bcl-XL expression, implicating its role in atherosclerosis and tumor survival .
Overexpression studies in prostate (DU145) and cervical (HeLa) cancer cells reveal PLC-β3's involvement in chemokine-mediated ERK activation and metastasis .

Neurological and Cardiovascular Systems

PLC-β3 is highly expressed in brain tissues, where it modulates G protein-coupled receptor (GPCR) signaling. Dysregulation correlates with neurological disorders and hypertension .

Cross-Reactivity and Specificity

Human Samples: Strong reactivity in HeLa, OVCAR3, and MCF7 cell lines .
Murine/Rat Samples: Detected in RAW 264.7 macrophages and brain tissues .
Specificity Controls: No cross-reactivity with PLC-β1, β2, or β4 isoforms .

Phosphorylation Studies

Anti-phospho-Ser537 antibodies confirm PLC-β3 activation in UV-treated MCF-7 cells and brain lysates, highlighting its role in stress responses .

Clinical and Therapeutic Implications

PLC-β3 antibodies have identified the enzyme as a potential biomarker for:

Myeloproliferative Disorders: Loss of PLC-β3 promotes HSC proliferation and MPN development .
Chemoresistance: Elevated PLC-β3 in ovarian (OVCAR3) and breast (MDA-MB-231) cancers correlates with poor prognosis .

Product Specs

Buffer

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

14-16 weeks (Made-to-order)

Synonyms

plc-3 antibody; T01E8.3 antibody; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma plc-3 antibody; EC 3.1.4.11 antibody; Phosphoinositide phospholipase C-gamma plc-3 antibody; Phospholipase C-gamma plc-3 antibody; PLC-gamma plc-3 antibody

Target Names

plc-3

Uniprot No.

Q22070

Target Background

Function

PLC-3 is a phospholipase C enzyme that plays a crucial role in intracellular signaling. It mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), key regulators of various cellular processes. Specifically, PLC-3's functions include: * **Regulation of Cell Contractions:** Controls basal and ovulatory sheath cell contractions by modulating Ca²⁺ oscillations through IP3-mediated activation of the IP3 receptor itr-1. * **Intestinal Function:** Regulates Ca²⁺ oscillations in intestinal epithelial cells, influencing posterior body wall muscle contractions essential for defecation. This involves IP3-mediated activation of itr-1 and likely activation of TRPM channels gon-2 and gtl-1 via PIP2 reduction. * **Antimicrobial Peptide Expression:** Activates tpa-1 via DAG production, thereby regulating the expression of the antimicrobial peptide nlp-29 in the epidermis in response to fungal infection or physical injury. * **Sensory Neuron Signaling:** Triggers Ca²⁺ transients via IP3-mediated activation of the IP3 receptor itr-1 in ASH sensory neurons, contributing to avoidance behavior elicited by nose touch. * **Neuronal Transmission and Molting:** Modulates neuronal transmission in ALA neurons, mediating the reduction in pharyngeal pumping and locomotion during the quiescent phase preceding larval molting. This process is downstream of lin-3 and the let-23 receptor and upstream of tpa-1, but independent of itr-1. * **Epidermal Morphogenesis:** May participate in epidermal morphogenesis during embryogenesis, potentially in conjunction with plc-1. * **Male-Sex Muscle Excitability:** Likely downstream of the daf-2 receptor, it regulates male-sex muscle excitability under conditions of food deprivation.

Database Links

KEGG: cel:CELE_T01E8.3

STRING: 6239.T01E8.3

UniGene: Cel.14497

Tissue Specificity

Expressed in intestine, isthmus of the pharynx, proximal gonad sheath cells, spermatheca and uterine sheath cells. In males, expressed in the valve cell, the vas deferens and retractor and ventral protactor muscles.

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plc-3 Antibody