PLDBETA1 Antibody

Biochemical Characteristics and Function

PLBD1 is a secreted protein with weak phospholipase activity, capable of hydrolyzing phospholipids such as phosphatidylcholine, phosphatidylinositol, and lysophospholipids . Structural studies indicate it contains a putative binding pocket that may function as an amidase or peptidase, though its precise catalytic mechanism remains under investigation .

Key functional attributes:

• Enzymatic activity: Exhibits phospholipase B-like activity but with lower efficiency compared to canonical phospholipases .

• Subcellular localization: Secreted into extracellular spaces, suggesting roles in intercellular signaling or lipid remodeling .

Species Homology and Genetic Information

PLBD1 is evolutionarily conserved across mammals, with notable homology in mice (100%), rats (92%), dogs (86%), and humans (79%) .

Genetic and protein identifiers:

Research Applications and Methodologies

PLBD1 antibodies are primarily used to study lipid metabolism and immune responses. For example:

• Western blotting: Detects PLBD1 in tissue lysates or serum samples .

• Immunoprecipitation: Isolates PLBD1 for functional studies on its enzymatic activity .

• Neutralization assays: Assesses the impact of PLBD1 inhibition on lipid-mediated signaling pathways .

Antibody fragmentation protocols (e.g., pepsin digestion) are often employed to generate antigen-binding fragments (F(ab')₂) for improved tissue penetration .