PLDN Human

Pallidin Homolog Human Recombinant
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Cat. No.
BT7722
Source
E.coli.
Synonyms
Pallidin protein homolog (mouse), PA, HPS9, PALLID, syntaxin 13-interacting protein pallid.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Introduction to PLDN Human

PLDN Human refers to recombinant pallidin, a protein encoded by the PLDN gene in humans. It plays a critical role in intracellular vesicle trafficking and the biogenesis of lysosome-related organelles, such as melanosomes and platelet-dense granules . This protein is a subunit of the BLOC-1 complex, which regulates vesicle-docking and fusion processes .

Molecular Structure and Properties

The recombinant PLDN Human protein is produced in E. coli and consists of a 192-amino acid polypeptide chain (1–172) with a molecular mass of 21.9 kDa. It includes a 20-amino acid His-tag at the N-terminus for purification .

PropertyDescription
Amino Acid SequenceMGSSHHHHHHSSGLVPRGSH... (full sequence provided in )
SourceE. coli
Purity>90% (SDS-PAGE)
Formulation20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 2 mM DTT, 10% glycerol
Storage4°C (short-term), -20°C (long-term)

Biological Function and Interactions

PLDN interacts with syntaxin-13 (STX12), a t-SNARE protein, to mediate vesicle fusion . It is part of the BLOC-1 complex, which includes:

  • CNO

  • Dysbindin (BLOC1S8)

  • MUTED

  • SNAPAP

  • BLOC1S1/S2 .

The protein is essential for:

  1. Lysosome-related organelle biogenesis (e.g., melanosomes, platelet-dense granules) .

  2. Intracellular membrane fusion via cooperation with syntaxin-13 .

Clinical and Pathological Relevance

Dysregulation of PLDN has been linked to:

ConditionMechanismSource
Hermansky-Pudlak Syndrome (HPS)Mutations in PLDN (e.g., HPS-9) disrupt granule biogenesis
Platelet-Dense Granule DeficiencyRUNX1 haplodeficiency reduces PLDN expression, impairing granule formation

Studies in erythroleukemia (HEL) cells show that PLDN downregulation mislocalizes dense granule markers (e.g., CD63) and reduces mepacrine uptake .

Antibodies and Assays

  • Antibody 10891-2-AP (Proteintech):

    • Target: PLDN (25 kDa observed)

    • Reactivity: Human, mouse, rat

    • Applications: WB, IP, IF, IHC, ELISA

Experimental Protocols

  • Western Blotting: Detects PLDN in lysates or purified protein samples .

  • Immunoprecipitation: Identifies interactions with BLOC-1 complex components .

Key Research Findings

  1. RUNX1-PLDN Axis:

    • RUNX1 binds to PLDN promoter regions, enhancing its transcription .

    • RUNX1 mutations reduce PLDN expression, leading to platelet granule defects .

  2. Structural Insights:

    • PLDN’s 20-amino acid His-tag facilitates affinity purification without altering function .

    • The protein’s stability in glycerol-containing buffers minimizes aggregation .

  3. Therapeutic Potential:

    • Dysregulation in PLDN may inform therapies for HPS and RUNX1-related disorders .

Product Specs

Introduction
Pallidin (PLDN) plays a crucial role in intracellular vesicle trafficking, collaborating with Syntaxin 13 to enable membrane fusion within cells. Several alternatively spliced transcript variations of the PLDN gene have been identified, but the complete structure of some variants remains to be fully elucidated. PLDN contributes to the formation of lysosome-related organelles, including melanosomes and platelet-dense granules. It interacts with various proteins such as Dysbindin, BLOC1S1, STX12, CNO, MUTED, SNAPAP, and BLOC1S2.
Description
Recombinant human PLDN, expressed in E. coli, is a single polypeptide chain with a molecular weight of 21.9 kDa. It consists of 192 amino acids, with the first 172 representing the PLDN sequence. A 20 amino acid His-tag is fused to the N-terminus to facilitate purification, which is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The provided PLDN solution has a concentration of 1 mg/ml and is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 2 mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the PLDN solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the PLDN protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Pallidin protein homolog (mouse), PA, HPS9, PALLID, syntaxin 13-interacting protein pallid.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSVPGPSSPD GALTRPPYCL EAGEPTPGLS DTSPDEGLIE DLTIEDKAVE QLAEGLLSHY LPDLQRSKQA LQELTQNQVV LLDTLEQEIS KFKECHSMLD INALFAEAKH YHAKLVNIRK EMLMLHEKTS KLKKRALKLQ QKRQKEELER EQQREKEFER EKQLTARPAK RM

Product Science Overview

Introduction

Pallidin homolog, also known as PLDN, is a protein that plays a crucial role in intracellular vesicle trafficking. This protein is encoded by the BLOC1S6 gene and is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules .

Structure and Characteristics

The recombinant human pallidin protein is typically produced in Escherichia coli (E. coli) and includes an N-terminal His-tag. The amino acid sequence of this recombinant protein corresponds to the amino acids 1-172 of the human pallidin protein . The theoretical molecular weight of the protein is approximately 21.9 kDa, although the observed molecular weight may vary due to post-translational modifications and other experimental factors .

Function and Interactions

Pallidin interacts with Syntaxin 13, a protein that mediates intracellular membrane fusion. This interaction is essential for the proper functioning of intracellular vesicle trafficking. The protein is also involved in the development of lysosome-related organelles, which are crucial for various cellular processes .

Applications

Recombinant human pallidin is used in various research applications, including:

  • Western Blotting: To detect the presence of pallidin in different cell lines.
  • Immunohistochemistry: To study the localization of pallidin in tissue samples.
  • Flow Cytometry: To analyze the expression of pallidin in different cell populations .
Storage and Handling

The recombinant human pallidin protein should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain the protein’s stability. The protein is typically supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 2 mM DTT, 10% glycerol, and 100 mM NaCl .

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