PLG Antibody, Biotin conjugated
Shipped with Ice Packs 
In Stock 
Description
Definition and Conjugation Process
PLG Antibody, Biotin Conjugated refers to a plasminogen-specific antibody chemically linked to biotin, a vitamin that forms high-affinity bonds with streptavidin or avidin. This conjugation process typically involves covalent attachment of biotin molecules to lysine residues or sulfhydryl groups on the antibody via NHS ester or maleimide chemistry . The resulting conjugate retains both the antibody's target specificity and biotin's utility for signal amplification.
Applications in Research
Biotin-conjugated PLG antibodies enable sensitive detection and quantification in diverse assays:
Research Findings and Performance Considerations
-
Biotin Density Impact: Higher biotin labeling density (e.g., 6 µg/mL vs. 2 µg/mL) correlates with increased antibody binding efficiency and accelerated target detection, though excessive labeling may impair antibody specificity .
-
Signal Amplification: Biotin-streptavidin systems enhance sensitivity by ~10–100x compared to direct enzyme conjugates, critical for low-abundance plasminogen detection .
-
Cross-Reactivity: Polyclonal biotin-conjugated antibodies (common for PLG) may exhibit broader species reactivity (e.g., human, mouse, rat) but require rigorous validation to minimize off-target binding .
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we are able to ship products within 1-3 business days after receiving your order. Delivery times may vary depending on the purchasing method or location. For specific delivery times, please consult your local distributors.
Synonyms
Plasmin antibody; Plasmin heavy chain A antibody; Plasmin light chain B antibody; Plasminogen antibody; PLG antibody; PLMN_HUMAN antibody
Target Names
Uniprot No.
Target Background
Function
Plasmin, a proteolytic enzyme, plays a crucial role in various biological processes, including the dissolution of blood clots, embryonic development, tissue remodeling, tumor invasion, and inflammation. It participates in ovulation by weakening the walls of the Graafian follicle and activates urokinase-type plasminogen activator, collagenases, and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Additionally, it cleaves fibrin, thrombospondin, and von Willebrand factor. CSPG4 may modulate its role in tissue remodeling and tumor invasion. Plasmin binds to cells. Angiostatin, an angiogenesis inhibitor, effectively blocks neovascularization and the growth of experimental primary and metastatic tumors in vivo.
Gene References Into Functions
- Apo(a) reduces cell-surface plasmin-mediated conversion of Glu- to Lys-plasminogen. PMID: 29990619
- Urinary angiostatin and VCAM-1 are predictive of specific histological changes in concurrent lupus nephritis renal biopsies. PMID: 29076253
- No association between the AgP risk variant rs4252120 and CP was found. However, a haplotype block downstream of PLG was identified and demonstrated shared association with CP and AgP. PMID: 28548211
- Homozygous alleles in F12 (rs1801020) and F13 (rs5985) were identified as a genetic risk profile of thromboembolism in a Family. PMID: 27976734
- The significant association of plasminogen and pSTAT3 with LI suggests that they may represent signaling nodes or biomarkers of pathways common to postlactational involution and LI. PMID: 28752190
- A rare non-conservative missense mutation was newly identified in exon 9 of the PLG gene. PMID: 29548426
- Plasminogen binds to the cell surface-exposed proteins of Candida parapsilosis. PMID: 28651026
- Plasmin cleaves surface-bound CCL21 to release the C-terminal peptide responsible for CCL21 binding to glycosaminoglycans on the extracellular matrix and cell surfaces, generating the soluble form. PMID: 27301418
- Analysis of plasminogen genetic variants in multiple sclerosis patients has been reported. PMID: 27194806
- Enolase of Mtb is present on its surface and binds human plasminogen with high affinity. PMID: 27569900
- The mechanism for plasminogen/M protein binding uncovered here may facilitate targeting of group A Streptococcus pyogenes virulence factors for disease management. PMID: 28724633
- t-PA binds to Lys91 in the MBP NH2-terminal region and PLG binds to Lys122 in the MBP COOH-terminal region. This proximity promotes the activation of PLG by t-PA. PMID: 28648598
- In the presence of platelet polyphosphate and the downstream substrate fibrin, alphaFXIIa is a highly efficient and favorable plasminogen activator. PMID: 27694320
- Plasmin(ogen) serves as a favorable biomarker for prediction of survival in advanced high-grade serous ovarian cancer. PMID: 27935848
- Our findings indicate a new pathway for bradykinin formation in patients with HAE, where FXII is cleaved and activated by plasmin. PMID: 27130860
- VWF susceptibility to plasmin proteolysis at K1491-R1492 is modulated by local N-linked glycan expression within A1A2A3, and specifically inhibited by heparin binding to the A1 domain. PMID: 28279966
- Bone morphogenetic proteins (BMPs) and mature BMPs that have been further cleaved by serum proteases induce cell cycle entry by dedifferentiating newt muscle cells. PMID: 28350991
- Plasminogen and P4HA2 are involved in vascular remodeling and angiogenesis, suggesting a high relevance of these processes for the pathogenic mechanisms underlying this type of vasculitis. PMID: 28041642
- Plasminogen and OxPL-PLG were lower in patients presenting with an acute MI than in those with stable CAD and also in those with atherothrombotic MI (Type 1) vs. those with non-atherothrombotic MI (Type 2). PMID: 26510751
- While carriers with PLG:p.Ala620Thr exhibit low plasminogen activity, this is not a predisposing variant for aHUS; and individuals with dysplasminogenemia are not at significantly increased risk of aHUS. PMID: 27194432
- Five novel plasminogen gene mutations have been found in Turkish patients with type I plasminogen deficiency. PMID: 26340456
- A novel plasminogen gene mutation, deficiency of plasminogen antigen and activity, and anti-plasminogen IgG and IgA antibodies were identified in a patient with adult-onset ligneous conjunctivitis. PMID: 25674820
- S. aureus NCTC 8325-4 adheres to immobilized plasminogen in vitro, and the adhesion may be mediated by a C-terminal fragment of the PBP3 protein [PBP3]. PMID: 27488131
- This study demonstrated that PLG functions as a molecular bridge between tricellulin and streptococcal surface enolase (SEN). The wild type strain efficiently translocated across the epithelial monolayer, accompanied by cleavage of transmembrane junctional proteins. PMID: 26822058
- Tubulointerstitial plasmin is associated with inflammation leading to renal fibrosis, and can cause the decline in renal function seen in patients with IgA nephropathy. PMID: 25971850
- Plasminogen binding and activation by different glycolytic enzymes of M. pneumoniae play a role in successful colonization of the human respiratory tract. PMID: 26667841
- Reduced proteolytic activity of plasmin on structures of growing thrombi, rather than on complement activation fragments, explains the association of plasminogen deficiency with aHUS. PMID: 26637181
- Zinc modulates fibrinolysis by attenuating tPA-mediated plasminogen activation and plasmin-induced fibrin degradation. PMID: 25789495
- These results indicate that FXIIIa activity can be modulated by fibrinolytic enzymes, and suggest that changes in fibrinolytic activity may influence cross-linking of blood proteins. PMID: 26359437
- Plasmin cleavage of iC3b provides a complement regulatory pathway that is as efficient as FI/CR1 but does not require a cellular cofactor. PMID: 25556624
- PLG is the third replicated shared genetic risk factor of atherosclerosis and periodontitis. PMID: 25466412
- Data show that preincubation with plasminogen, wild-type group A Streptococcus (GAS) NS88.2 degraded complement C3b. PMID: 23969887
- While the presence of plasminogen did not affect the factor I cofactor activity of C4BP, the activation of plasminogen by urokinase-type plasminogen activator to active plasmin was significantly augmented in the presence of C4BP. PMID: 26067271
- These studies demonstrate that GAS virulence can be explained by disparate hPg activation by SK2a and SK2b coupled with the coinherited M-proteins of these strains. PMID: 26070561
- PAM activated Plasminogen Glycoform II. PMID: 26029848
- High plasma fibrinogen and low plasminogen are associated with poor survival in CTEPH patients without modern therapy. PMID: 24909805
- Data show that different subpopulations of platelets harbor plasminogen by diverse mechanisms. PMID: 25712989
- Manganese transport protein C (MntC) is an extracellular matrix- and plasminogen-binding protein. PMID: 25409527
- This review highlights the importance of the best-characterized components of the PLG/PLA cascade in the pathogenesis of cancer focusing on the role of the cell surface-PLG receptors (PLG-R). [review] PMID: 25407528
- IGF-II, TGF-beta1 and VEGF-A and its receptor in malignant tumor tissue, as well as increased plasmin release from proenzyme and MMP-3 activation, are apparently associated with the formation of pathogenic mechanism of vasculature development. PMID: 25993872
- Angiostatin may play a role in the pathophysiology of preeclampsia. PMID: 24205998
- The results suggested that EF-Tu and Eno serve as surface receptors for B. longum NCC2705 binding to human plasminogen. PMID: 24840471
- Human plasmin activity loss results from the C-terminal lysine-dependent redistribution of enzyme molecules on a fibrin surface. PMID: 25222106
- Genome-wide association analyses revealed common DNA variants in PLG, LPA, and near SIGLEC14 that contribute to plasma plasminogen level variation. PMID: 25208887
- ANG interacts with plasminogen activation system at the leading edges of breast cancer cell surfaces and facilitates interactions of uPAR with uPA to regulate plasmin formation and cell migration. PMID: 24457100
- Reduced plasminogen binding and delayed activation render gamma'-fibrin more resistant to lysis than gammaA-fibrin. PMID: 25128532
- Binding of streptokinase Lys(414) to plasminogen kringle 4 plays a role in recognition of plasminogen by streptokinase. PMID: 25138220
- The surface-displayed enolase, which serves as major pneumococcal plasminogen receptor, was identified as a key factor for plasminogen-mediated bacterial attachment in infection analyses with Streptococcus pneumoniae. PMID: 23906818
- The results demonstrate that Bacteroides fragilis Bfp60 surface adhesin is responsible for the recognition of laminin and plasminogen-plasmin activation. PMID: 23850366
- We propose that plasminogen activation on endothelial cells acts as a natural backup for ADAMTS13 to degrade obstructive platelet-VWF complexes. PMID: 24449821
Database Links
Involvement In Disease
Plasminogen deficiency (PLGD)
Protein Families
Peptidase S1 family, Plasminogen subfamily
Subcellular Location
Secreted. Note=Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
Tissue Specificity
Present in plasma and many other extracellular fluids. It is synthesized in the liver.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
