plk-1 Antibody
Description
PLK1 Antibody Overview
PLK1 antibodies detect the human PLK1 protein (UniProt ID: P53350), which regulates centrosome maturation, spindle assembly, and cytokinesis . These antibodies are widely used in techniques such as Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), and flow cytometry (FC) .
Cancer Biology
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Mechanistic Insights: PLK1 is overexpressed in cancers (e.g., lung adenocarcinoma, breast cancer) and promotes tumor growth by activating biosynthesis pathways like the pentose phosphate pathway via G6PD phosphorylation .
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Therapeutic Targeting:
HIV-1 Pathogenesis
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PLK1 stabilizes HIV-1-infected CD4+ T cells by enhancing SUMOylation, enabling viral reservoir persistence. Dual PLK1-BET inhibitors (e.g., UMB-151) reduce latent HIV reservoirs while reactivating virus .
DNA Methylation Regulation
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PLK1 inhibition reduces global DNA methylation by destabilizing UHRF1, a key epigenetic regulator, leading to tumor suppressor gene re-expression .
Prognostic Marker
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High PLK1/p-PLK1 expression correlates with poor survival in NK/T cell lymphoma (5-year OS: 71.1% vs. 29.6% in low vs. high PLK1 groups) .
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PLK1 overexpression in breast cancer associates with TP53 mutations and triple-negative subtypes .
Immunotherapy Synergy
Technical Considerations
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Validation: Antibodies like 10305-1-AP and 3F8 show specificity via single-band WB at ~68 kDa .
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Storage: Most antibodies require storage at -20°C in PBS with sodium azide and glycerol .
Challenges and Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
PLK-1, a serine/threonine kinase, plays a critical role in regulating various aspects of cell division in the nematode *Caenorhabditis elegans*. It is essential for the proper execution of key events, including:
- Oocyte Nuclear Envelope Breakdown: PLK-1 is required for the breakdown of the nuclear envelope in oocytes prior to their entry into the spermatheca, a crucial step in the meiotic process.
- Spindle Dynamics and Attachment: In meiotic cells, PLK-1 plays a vital role in regulating spindle dynamics and ensuring proper attachment of the spindle to chromosomes.
- Germline and Nerve Cord Development: PLK-1 is essential for the development of the germline and nerve cord during embryonic development.
- Spindle Organization and Centrosome Maturation: In mitotic cells, PLK-1 participates in spindle organization and centrosome maturation, ensuring accurate chromosome segregation.
- Asymmetric Nuclear Localization of CDC-25.1: PLK-1 is involved in the asymmetric localization of CDC-25.1 during embryogenesis, which influences cell division timing.
- Cytoplasm Polarity Regulation: In collaboration with PLK-2, PLK-1 regulates cytoplasm polarity in early embryos, contributing to proper cell development.
- Potential Role in Chromosome Pairing and Synapsis: PLK-1 may play a minor role in chromosome pairing and synapsis during oocyte meiosis I.
Further research highlights the crucial and conserved role of PLK-1 in various cellular processes:
- PLK1 inhibition has been shown to mitigate autophagy in cancer cells, both under nutrient starvation and sufficiency. This finding indicates a conserved role for PLK1 in autophagy, as observed in the invertebrate model organism *C. elegans*. PMID: 28102733
- Studies have revealed that phosphorylation of the N-terminus of Bora is critical for Plk1 activation and mitotic entry. PMID: 27068477
- PLK-1 has been identified as essential for nuclear envelope breakdown during early embryogenesis. PMID: 26490119
- PLK-1 has been found to substitute for Mps1 in controlling spindle checkpoint initiation in *C. elegans*. PMID: 26119738
- NCAPG2 plays a significant role in regulating proper chromosome segregation through a functional interaction with PLK1 during mitosis. PMID: 25109385
- Research has provided insights into the regulation of homolog pairing and revealed that the targeting of plk-1 to the nuclear envelope by meiotic chromosomes establishes the conserved linkages to cytoskeletal forces necessary for homology assessment. PMID: 22018921
- Asymmetry in plk-1 contributes to asynchronous cell division in *C. elegans* embryos. PMID: 18305005
Customer Reviews
Applications : Western blot
Sample type: Human Cells
Review: Western blot assays showed that the tBHP and SAHA-induced suppression of phospho-FOXM1, AURKA and PLK1, as well as phospho-CCNB1.
