At1g79820 Antibody

Functional Role of the Target Protein (SGB1)

• Genetic Interaction: SGB1 acts synergistically with the heterotrimeric G-protein β-subunit (AGB1) to regulate early seedling development .

• Subcellular Localization: Golgi apparatus .

• Transport Activity: Facilitates hexose transport, critical for sugar distribution during plant growth .

• Mutant Phenotypes: Impaired growth under low-sugar conditions, highlighting its role in nutrient sensing .

Key Validation Data

• Specificity: Recognizes a single band at ~52 kDa in Western blots of Arabidopsis extracts, consistent with SGB1’s predicted size .

• Cross-Reactivity: Detects homologues in Brassica napus but not confirmed in other species .

• Functional Assays: Used to study SGB1’s role in Golgi-mediated sugar transport and seedling development .

Research Applications

• Protein Localization: Confirmed Golgi localization via immunocytochemistry .

• Gene Expression Studies: Paired with promoter-GFP/GUS constructs to analyze tissue-specific At1g79820 expression .

Critical Considerations for Use

• Specificity Challenges: Commercial antibodies for plant proteins often exhibit cross-reactivity . While SGB1 antibody validation data exist , independent verification using At1g79820 knockout lines is recommended.

• Storage Stability: Lyophilized form ensures long-term stability but requires careful reconstitution to avoid degradation .

Comparative Analysis of Available Antibodies

Research Findings and Implications

• Sugar Signaling: SGB1 knockdown mutants show altered responses to exogenous glucose, linking it to sugar-sensing pathways .

• Developmental Defects: Impaired seedling growth in mutants underscores SGB1’s role in nutrient allocation .

• Biotechnological Potential: Engineering SGB1 variants could enhance stress tolerance in crops by optimizing sugar transport .

Future Directions

• Structural Studies: Resolving SGB1’s 3D architecture to elucidate substrate-binding mechanisms.

• CRISPR-Based Validation: Generating At1g79820 knockout lines to confirm antibody specificity .

• Agricultural Applications: Modulating SGB1 expression to improve crop yields under nutrient-limited conditions.