plx-2 Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
PLX-2 acts as a receptor for mab-20/sema-2a, playing a crucial role in the formation and stabilization of cell-cell connections during various stages of epithelial morphogenesis. In early embryonic development, PLX-2 is essential for the proper ventral closure of the epidermis. During male tail morphogenesis, it contributes to precursor cell sorting and the formation of distinct sensory rays. Furthermore, PLX-2 is involved in the guidance of SDQL neurons during neurogenesis. It is believed that upon stimulation by mab-20, PLX-2 regulates fln-1-mediated remodeling of the actin cytoskeleton, thereby influencing axon guidance and/or fasciculation of DD/VD neurons.
- PLX-2 plays a significant role in the cell movements required for ventral enclosure in C. elegans. PMID: 22197242
Q&A
The following FAQs address key methodological considerations for researchers using PLCG2 (phospholipase C gamma 2) antibodies in academic settings, combining technical validation data and experimental design insights from peer-reviewed protocols.
Advanced Research Questions
How to resolve conflicting PLCG2 expression data across studies?
How to design a co-IP protocol for PLCG2 interaction studies?
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Lysis conditions: Use mild detergents (e.g., 1% NP-40) to preserve protein complexes .
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Antibody coupling: For ab248535, couple 1–5 µg antibody to Protein A/G beads per 100 µg lysate .
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Elution: Validate with SDS-PAGE and Western blot for co-precipitated partners (e.g., BTK or SYK) .
What are the pitfalls in quantifying PLCG2 phosphorylation?
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Dynamic phosphorylation: Time-course experiments (e.g., 0–30 min post-stimulation with pervanadate) are essential to capture transient states .
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Normalization: Use total PLCG2 (e.g., ab133522) as a loading control alongside phospho-specific antibodies (e.g., MAB37161) .
