PME26 Antibody

Product Overview

The PME26 antibody (Product Code: CSB-PA864856XA01DOA) is a rabbit-derived polyclonal antibody targeting the Arabidopsis thaliana PME26 protein. Key specifications include:

3.1. Western Blot and ELISA

The PME26 antibody validates protein expression in Arabidopsis tissues, with optimized dilutions of 1:500–1:2000 for WB . Its reactivity is confirmed in ELISA, making it suitable for quantitative assays .

3.2. Functional Studies

While PME26-specific data are sparse, studies on PME mutants highlight methodologies applicable to PME26:

• Activity Assays: Total PME activity is measured in plant extracts post-pathogen challenge .

• Mutant Analysis: Double/triple PME mutants (e.g., pme31 pme52) show compromised immunity, suggesting PME26 could contribute similarly .

4.1. Pathogen-Induced PME Activity

• PME activity increases significantly (e.g., 72 hours post-P. syringae infection), enhancing cell wall remodeling .

• Mutants like pme3 exhibit reduced baseline activity but heightened induction during infection .

4.2. Genetic Redundancy

• Phylogenetic Grouping: PMEs cluster into functional groups (e.g., Groups A and D). Mutants in Group D (e.g., pme35, pme39) show additive susceptibility phenotypes .

• Epistatic Interactions: Double mutants (e.g., pme12 pme22) display synergistic effects, underscoring functional overlap .

Limitations and Future Directions

• Specificity Gaps: PME26’s exact role in immunity remains uncharacterized compared to other PMEs.

• Antibody Validation: Further studies are needed to confirm cross-reactivity and optimize protocols for diverse plant species.