PMEI6 Antibody
Product Specs
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Studies on pme6-1 mutant guard cells reveal walls enriched in methyl-esterified pectin. These cells exhibit a reduced dynamic response to stomatal opening/closure triggers (e.g., increased osmoticum). This impaired stomatal function, attributed to altered guard cell wall mechanics, results in increased conductance, evaporative cooling, and reduced plant growth. (PMID: 27720618)
- PMEI6 effectively inhibits endogenous pectin methylesterase (PME) activity. (PMID: 23362209)
Q&A
Here’s a curated collection of FAQs for PMEI6 antibody in academic research, structured by complexity and grounded in experimental evidence:
How should PMEI6 antibody specificity be validated in mutant backgrounds?
Methodological approach:
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Use pmei6 knockout mutants (e.g., pmei6-1) as negative controls .
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Compare with 35S:PMEI6 overexpression lines for antibody signal intensity .
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Combine with enzymatic activity assays (e.g., PME inhibition tests) to correlate antibody labeling with functional outcomes .
What experimental designs resolve contradictions in PMEI6’s role across studies?
Conflicting data often arise from tissue-specific PMEI6 expression or pathogen/pest models. Advanced strategies:
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Contextual phenotyping: Compare mucilage release (seed coat) vs. pathogen response (leaves) in the same mutant .
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HG methylation profiling: Use combinatorial antibody panels (e.g., LM20 for high esterification, LM19 for low esterification) to map spatial patterns .
Example contradiction: PMEI6 overexpression enhances Botrytis resistance but has no effect on aphid susceptibility. Resolution involves:
How does PMEI6 antibody aid in studying cell wall integrity trade-offs?
PMEI6 regulates a balance between wall rigidity (via peroxidase anchoring) and flexibility (via pectin de-methylesterification). Key methodologies:
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Immunolocalization: Track PRX36 recruitment using LM20-labeled HG domains .
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Biomechanical assays: Measure mucilage extrusion force in pmei6 vs. wild-type seeds .
| Parameter | Tool/Assay | Outcome in pmei6 Mutants |
|---|---|---|
| HG methylesterification | JIM7/LM20 labeling | Absent in cell wall fragments |
| Wall brittleness | Seed hydration time-lapse | Delayed mucilage release |
What advanced techniques complement PMEI6 antibody data?
Integrate antibody-based observations with:
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Transcriptomics: Analyze PMEI6 expression under GLABRA2 or MYB52 regulation .
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Enzyme kinetics: Measure PMEI6’s inhibitory constant (Kᵢ) against PME isoforms .
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Structural biology: Use homology modeling to predict PMEI6-PME interaction sites .
