PML39 Antibody

Introduction

The PML39 antibody targets the Saccharomyces cerevisiae protein PML39, a 39-kDa nuclear periphery protein critical for pre-mRNA retention and quality control. Initially identified through genetic screens, PML39 interacts with nucleoporins (e.g., Mlp1/2) and regulates improper mRNA retention prior to export . This article synthesizes data on PML39 antibody characteristics, applications, and research findings.

Technical Details

• ARP39753_P050: Provided in 1x PBS with sodium azide and sucrose; validated via Western blot .

• ab96051: Targets sumoylated isoforms (PML-4, -12) linked to antiviral functions .

• A00093: Reacts with the N-terminal domain; optimized for IF and IHC (1:50-1:200 dilution) .

Role in mRNA Retention

PML39 anchors to nuclear pore complexes (NPCs) via Mlp1/2 proteins, forming a retention pathway for unspliced mRNAs . Key findings:

• mRNA leakage: pml39Δ mutants exhibit unspliced mRNA leakage, enhanced by Rrp6 (exosome subunit) deletion .

• Overexpression effects: Pml39-GFP overexpression traps Nab2 (hnRNP) and intron-containing mRNAs in nuclear foci .

Interactions and Pathways

• Mlp proteins: PML39 interacts with Mlp1/2 N-terminal domains, mediating Nab2 recruitment .

• mRNP assembly: Deletion of PML39 rescues thermosensitive phenotypes in mRNP mutants (e.g., yra1-8), indicating bypass of quality control .

Research Focus Areas

1. mRNA export regulation: Studies on PML39's role in retaining unspliced mRNAs .

2. NPC dynamics: Localization of PML39 in nuclear foci during stress .

3. Antiviral mechanisms: Cross-reactivity with human PML isoforms (e.g., PML-4) in viral sequestration .

Experimental Uses

• Western blot: Detects PML isoforms in nuclear fractions .

• Immunofluorescence: Visualizes PML39 localization at NPCs .

• Immunohistochemistry: Analyzes PML expression in cancer tissues .