PNP2 Antibody

Overview of PNPLA2 Antibody

PNPLA2 antibodies are immunological tools designed to detect and study the PNPLA2 protein, a 54 kDa transmembrane enzyme critical for lipid metabolism. These antibodies are used in research to investigate PNPLA2's roles in adipose tissue lipolysis, retinal function, and cancer biology .

Antibody Structure

• Format: Monoclonal (e.g., MAB5387) or polyclonal (e.g., AF5387) .

• Epitope Recognition: Binds to the immunogen region spanning Val162-Thr332 in human PNPLA2, which shares 85% amino acid identity with mouse and rat homologs .

• Conjugates: Available in unconjugated or Alexa Fluor® 405-conjugated formats for enhanced detection in imaging .

Target Protein Function

• Catalytic Activity: Hydrolyzes triglycerides into diacylglycerol in adipose tissue .

• Tissue Distribution: Highly expressed in adipose tissue, retina, testis, and cardiac/skeletal muscle .

Research Applications

PNPLA2 antibodies are validated for:

Cancer Therapeutics

• Combination Therapy: In a murine Lewis lung carcinoma model, PNPLA2 antibodies were used alongside pigment epithelium-derived factor (PEDF) gene therapy, demonstrating enhanced tumor suppression .

Metabolic Studies

• Lipolysis Regulation: PNPLA2 knockout models show impaired triglyceride breakdown, linking its activity to obesity and metabolic disorders .

Validation and Quality Control

• Specificity: Validated via siRNA knockdown and immunoblotting .

• Storage: Stable at -20°C to -70°C; avoid repeated freeze-thaw cycles .

Limitations and Considerations

• Cross-Reactivity: Limited data on non-mammalian species .

• Clinical Relevance: While PNPLA2 is implicated in metabolic diseases, therapeutic antibodies remain experimental.