PNRC1 Antibody
Description
Definition and Function
The PNRC1 antibody is a polyclonal rabbit IgG antibody designed to detect human PNRC1 (Proline-Rich Nuclear Receptor Coactivator 1), a tumor suppressor protein. PNRC1 regulates cellular proliferation by inhibiting rRNA maturation through interactions with the DCP1α/DCP2 decapping machinery, thereby blocking oncogene-driven growth .
Applications and Dilution Recommendations
| Application | Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1000 |
| ELISA | Varies by assay |
| Immunohistochemistry (IHC) | 1:20–1:50 |
| IHC-Paraffin | 1:20–1:50 |
Note: Optimal dilutions may require titration per experimental system .
Research Findings
Tumor Suppression Mechanism
PNRC1 acts as a tumor suppressor by:
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Blocking oncogene-induced proliferation: Co-expression of PNRC1 with HRAS G12V or MYC oncogenes completely abolishes their proliferative effects in HeLa and MCF7 cells .
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Inhibiting rRNA maturation: PNRC1 recruits the DCP1α/DCP2 decapping machinery to the nucleolus, leading to accumulation of unprocessed 47S pre-rRNA precursors and reduced mature 28S rRNA .
Expression and Localization
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PNRC1 is downregulated in cancers (e.g., breast, lung) compared to normal tissues .
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Its expression is mutually exclusive with the proliferation marker Ki-67, suggesting a role in quiescent cells .
Published Studies
Product Specs
Target Background
- PNRC1 and Grb2, through their interaction, can suppress nuclear receptor-mediated regulation and growth factor-mediated regulation in human breast tissue. PMID: 15122321
- Deletion mutagenesis, transient transfection, and luciferase assays revealed that the -123/+27 region is the minimal promoter of the human PNRC1 gene. PMID: 18281297
- Three human PNRC1 splicing variants, designated PNRC1c, PNRC1d, and PNRC1f, have been identified. PMID: 18703122
- RFX1 specifically binds to the promoter region and negatively regulates the transcription of the human PNRC1 gene. PMID: 19334528
