PNSL2 Antibody
Description
Contextual Identification of PnsL2 in Plant Biology
The term "PnsL2" appears in structural studies of the chloroplast NDH-PSI supercomplex, which facilitates cyclic electron flow in photosynthesis. Key insights include:
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Role in NDH Assembly: PnsL2 (alongside PnsB2 and PnsB3) stabilizes the membrane arm of the NDH complex, critical for interactions with photosystem I (PSI) .
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Structural Features: Cryo-EM studies reveal PnsL2’s β-sheet-rich architecture and its role in mediating subunit interactions within the NDH-PSI supercomplex .
Antibodies Targeting NDH-Related Proteins
While no commercial antibodies specific to PnsL2 are documented, polyclonal antibodies against analogous plant proteins are widely used. Examples from Agrisera’s catalog include:
| Antibody ID | Target Protein | Reactivity | Applications |
|---|---|---|---|
| AS16 4030 | PSI Subunit PsaA | Arabidopsis thaliana | WB, IF |
| AS15 3060 | NDH Subunit NdhF | Arabidopsis thaliana | WB, ELISA |
| AS14 2772 | Calvin Cycle Enzymes | Arabidopsis, Oryza | WB, IP |
Source: Agrisera antibody database
Research Applications of Plant Protein Antibodies
Antibodies targeting chloroplast proteins are pivotal for:
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Proteomic Studies: Quantitative Western blotting and immunoprecipitation (IP) to analyze protein expression under stress conditions .
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Structural Biology: Validating knockout (KO) lines for cryo-EM and crystallography, as demonstrated in NDH-PSI supercomplex mapping .
Validation Challenges and Best Practices
Recent initiatives emphasize rigorous antibody validation:
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Multi-Application Testing: Antibodies for plant proteins are screened via Western blot (WB), immunofluorescence (IF), and IP, with KO controls to confirm specificity .
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Case Study: A 2023 eLife study validated 65 antibodies for neurological and plant targets, achieving 85% specificity in WB and 62% in IF .
Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- PQL2 protein levels were elevated in mutant plants exhibiting deficiencies in the PSI complex or the thylakoid membrane-bound ferredoxin-NADP(+) oxidoreductase. PMID: 20460499
