pou5f1 Antibody
Description
Introduction
The POU5F1 antibody targets the POU5F1 protein, a transcription factor critical for embryonic stem cell pluripotency and self-renewal. Its role extends to cancer stem-like cells (CSLCs), where it promotes tumorigenesis and metastasis. This article synthesizes data on antibody specifications, applications, and research findings, drawing from diverse sources .
Definition and Function of POU5F1
POU5F1, also known as OCT4, is a homeodomain transcription factor encoded by the POU5F1 gene. It regulates genes like NANOG and UTF1 to maintain embryonic stem cell (ESC) pluripotency . In cancer, POU5F1 drives CSCL self-renewal and metastasis by upregulating matrix metalloproteinases (MMPs) and activating epithelial-to-mesenchymal transition (EMT) .
Applications of POU5F1 Antibody
The antibody is used in:
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Western Blot (WB): Detects POU5F1 expression in cancer cells and ESCs .
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Immunoprecipitation (IP): Identifies POU5F1 interactions with transcriptional partners (e.g., SOX2) .
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Immunocytochemistry (ICC): Visualizes nuclear localization in stem cells .
Role in Cancer
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Tumor Growth: POU5F1 overexpression enhances proliferation and metastasis in gastric cancer (GC) , lung adenocarcinoma , and sarcomas .
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Mechanisms:
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Therapeutic Targeting: All-trans retinoic acid (ATRA) inhibits POU5F1-induced EMT and metastasis .
Antibody-Validated Studies
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Knockdown Experiments: POU5F1 siRNA reduces CSCL invasion and induces senescence .
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Biomarker Potential: Co-expression of POU5F1 and MMP-2 correlates with poor prognosis in lung adenocarcinoma .
Clinical Implications
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- POU2 is essential for endoderm formation. Embryos lacking maternal and zygotic POU2 exhibit a deficiency in endodermal precursors. PMID: 14723850
- Western blot analysis conducted on developing hatchlings using Oct4 antibody at 13.6 and 21.6mugL(-1) doses revealed overexpression, suggesting a stimulatory role of nanosilver. In silico analysis demonstrated a strong interaction between nanosilver and Oct4, highlighting their crucial role in stimulating growth during embryonic development. PMID: 29778081
- Research indicates that Pou5f3 forms complexes with Nanog at the single-cell level in vivo, contributing to mesendoderm differentiation pattern during the blastula stage. PMID: 27684073
- Studies suggest that Znfl1 (zinc finger-like gene 1) regulates the developmental gene expression of Hoxb1b (homeobox B1b protein) in the embryonic posterior neuroectoderm by acting upstream of Pou5f3 (POU domain class 5 transcription factor 3) and Sall4 (spalt-like transcription factor 4); these proteins appear to be involved in neurogenesis. PMID: 28623229
- Data suggest that the regulation of mych by Pou5f1 is achieved through direct transcriptional activation. PMID: 24643012
- Posttranslational modification by phosphorylation suggests that Pou5f1's activity or stability may be subject to temporal or region-specific modulation by embryonic signaling mechanisms. PMID: 24130110
- Research delves into the mechanistic implications of simultaneous activation of transcriptional targets by ubiquitous factors, like Pou5f1, and region-specific inducers, emerging as a common regulatory motif in early development. PMID: 24211655
- Maternal Nanog, Pou5f1, and SoxB1 are essential for initiating the zygotic developmental program and inducing clearance of the maternal program by activating miR-430 expression. PMID: 24056933
- These findings place Pou5f1 and SOX-POU sites at the heart of the zygotic gene activation network in vertebrates, providing a link between zygotic gene activation and pluripotency control. PMID: 23950494
- The defects caused by HEP induction were rescued by introducing wild-type pou2 mRNA before the heat treatments. PMID: 22921661
- Vox plays a crucial role downstream of BMP signals in regulating the capacity of Nodal to induce endoderm versus mesoderm by modulating the activity of the Casanova/Pou2 regulatory system. PMID: 23364327
- Pou2 participates in multiple aspects of vertebrate development, particularly in the binary decision of the mesendoderm to mesoderm and endoderm, employing different mechanisms depending on the developmental stage. PMID: 22913532
- Research demonstrates that Pou5f1 binds to phylogenetically conserved Oct/Pou5f1 sites in the vox promoter, both in vivo and in vitro. PMID: 21621531
- The temporospatial structure of the zebrafish Pou5f1 target networks may explain aspects of the evolution of mammalian stem cell networks. PMID: 20212526
- Data indicate that embryos genetically depleted of both maternal and zygotic pou2 function exhibit severe dorsoventral patterning defects and, independently, a blastoderm-specific arrest of epiboly. PMID: 16775002
- Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defects observed in Pou5f1/Oct4 deficient MZspg zebrafish embryos. PMID: 18215655
- These findings provide a model where pou2/pou5f1 expression in zebrafish embryos is regulated by both an autoregulatory loop and repression by RA originating from the posterior mesoderm. PMID: 18407549
