POLR2E Antibody
Description
Structure and Function of POLR2E
POLR2E is a shared subunit among RNA polymerases I, II, and III, contributing to their structural and functional stability. It is part of the lower jaw domain of Pol II, which binds the DNA template during transcription . The gene encoding POLR2E is located on human chromosome 19 and has a pseudogene on chromosome 11 .
| Feature | Description |
|---|---|
| Molecular Weight | 25 kDa |
| Gene Locus | Chromosome 19q13.33 |
| Interactions | Binds transcription factors (e.g., TAF15) and other Pol II subunits |
Medical Relevance: Autoimmune Responses
POLR2E is implicated in autoimmune diseases like scleroderma, where autoantibodies target RNA polymerase II. Research indicates that the carboxyl-terminal domain (CTD) of Pol II’s largest subunit contains epitopes recognized by these autoantibodies . POLR2E antibodies are used in diagnostic assays to detect such autoimmune markers .
Research Applications
POLR2E antibodies are employed in:
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Transcriptional Studies: Investigating RNA Pol II dynamics and chromatin interactions .
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Disease Diagnosis: Detecting scleroderma-associated autoantibodies .
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Subcellular Localization: Identifying Pol II in nucleus and cytoplasm via IHC/IF .
Key Research Findings
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Autoimmune Epitopes: The CTD of Pol II’s largest subunit is a dominant target for autoantibodies in scleroderma .
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Expression Patterns: POLR2E is ubiquitously expressed in human tissues, with high levels in kidney and liver .
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Therapeutic Potential: Inhibiting POLR2E interactions may modulate transcriptional activity in cancer or autoimmune conditions .
References:
Product Specs
Target Background
- The POLR2E rs3787016T allele was associated with an increased cancer risk in the Chinese population. PMID: 29724531
- The POLR2E rs3787016 C/T and HULC rs7763881 SNPs were associated with a significantly decreased risk for ESCC (p < 0.005). The ANRIL rs2151280 T/C SNP was not associated with the risk of ESCC. PMID: 25874495
- ELL plays an early and essential role during rapid high-amplitude gene expression, which is required for both Pol II pause site entry and release. PMID: 22252557
- The crystal of RPB5 diffracted to 2.8 Å resolution and belonged to space group P4(3)2(1)2. PMID: 22102239
- RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells. PMID: 21310960
- Results pinpoint critical residues for RNA polymerase II subunit 5 binding to RAP30 and/or to HBx, and identify these residues in both mammalian cells and in an in vitro binding assay. PMID: 16169872
