POMP Antibody
Description
Definition and Target Specificity
The POMP antibody is designed to detect the human proteasome maturation protein (UniProt ID: Q9Y244), a 16 kDa protein essential for coordinating proteasome biogenesis. It facilitates α-ring formation, β-subunit recruitment, and ER-associated assembly of 20S core complexes . The antibody is validated for use in Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF/ICC) .
Table 1: Validated Applications of POMP Antibody (15046-1-AP)
| Application | Dilution Range | Detected In |
|---|---|---|
| Western Blot | 1:1,000–1:4,000 | HeLa, HepG2, L02 cells |
| IHC | 1:20–1:200 | Human liver, skin tissue |
| IF/ICC | 1:200–1:800 | HepG2, HeLa cells |
This antibody has been utilized to:
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Track POMP localization at the endoplasmic reticulum during proteasome assembly .
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Study IFN-γ-induced immunoproteasome formation by detecting POMP-LMP7 interactions .
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Monitor POMP degradation as a marker of proteasome maturation .
Role in Immunoproteasome Biogenesis
POMP is upregulated by IFN-γ to accelerate immunoproteasome (i20S) assembly. It recruits β5i (LMP7) subunits, enabling rapid antigen processing for MHC-I presentation. Silencing POMP reduces LMP7 incorporation, impairing proteasome activity and MHC-I surface expression .
Impact of POMP Silencing
Table 2: Consequences of POMP Knockdown
| Parameter | Effect Observed |
|---|---|
| Proteasome subunits | ↓ α7 and β5 levels (cell-type dependent) |
| ER stress | ↑ BiP and CHOP expression |
| Cell viability | Induces apoptosis |
In epidermal tissue, POMP depletion mimics KLICK genodermatosis, causing aberrant proteasome subunit distribution and ER stress .
Technical Considerations
Product Specs
Target Background
- A novel disease termed POMP-related autoinflammation and immune dysregulation disease (PRAID) has been identified in two unrelated individuals. This disease is characterized by a unique combination of early-onset combined immunodeficiency, inflammatory neutrophilic dermatosis, and autoimmunity. PMID: 29805043
- POMP is overexpressed and contributes to increased proteasome assembly and activity in psoriatic skin. PMID: 28728908
- Mutations in MCM3AP and POMP have been linked to a DNA-repair and DNA-damage-signaling defect in an immunodeficient child. PMID: 26615982
- Research supports a role for the NRF2/POMP axis in bortezomib resistance. NRF2 and POMP are identified as potential targets for chemosensitization to this proteasome inhibitor. PMID: 26483548
- MicroRNA-101 (miR-101) targets the proteasome maturation protein POMP, leading to impaired proteasome assembly and activity. This results in the accumulation of p53 and cyclin-dependent kinase inhibitors, cell cycle arrest, and apoptosis. PMID: 26145175
- Single-nucleotide polymorphisms in the POMP gene have been associated with breast cancer risk following menopausal hormone replacement therapy. PMID: 24080446
- KLICK is characterized by reduced levels of POMP, leading to proteasome insufficiency in differentiating keratinocytes. PMID: 22235297
- A single-nucleotide deletion in the POMP 5' UTR causes a transcriptional switch and altered epidermal proteasome distribution in KLICK genodermatosis. PMID: 20226437
- POMP/proteassemblin/hUmp1 may exist as a tetramer and is localized to both the cytoplasm and the nucleus. PMID: 16624403
- Research indicates that POMP facilitates key steps in 20S core complex formation at the ER, coordinating the assembly process and ensuring the availability of newly formed proteasomes at their functional site. PMID: 17948026
