Customize At1g63150 Antibody

Description

Biological Context and Functional Associations

The At1g63150 gene product belongs to a family of proteins implicated in transcriptional regulation. Experimental data from chromatin immunoprecipitation (ChIP) studies demonstrate its involvement in leaf development processes through interactions with MYB-domain transcription factors . Specifically:

Validated in ChIP-PCR experiments showing DNA-protein binding activity
Associated with pathways regulating cell proliferation and organ size determination
Co-expressed with genes involved in auxin signaling and cell cycle regulation

Notably, the antibody helped identify binding patterns at promoter regions of developmental regulators like ANT and CYCD3;1 .

Research Applications and Validation Status

While commercial documentation confirms basic reactivity , independent validation data remain limited compared to mammalian antibody standards. Key considerations:

Demonstrated specificity in Arabidopsis protein extracts
Used successfully in chromatin studies showing differential binding during developmental stages
No published cross-reactivity tests with orthologous proteins from other plant species

Comparative analysis with other Arabidopsis antibodies reveals:

Similar technical validation protocols to PCMP-H44 and POT5 antibodies
Shared applications in studying plant-specific chromatin modifiers

Research Implications and Future Directions

Current applications focus on:

Mapping protein-DNA interactions in developmental biology
Characterizing auxin-responsive gene networks
Comparative studies with other plant model systems

Critical research gaps include:

Structural characterization of the target epitope
In planta functional knockout correlation studies
Cross-species reactivity profiling

Product Specs

Buffer

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4

Form

Liquid

Lead Time

14-16 week lead time (made-to-order)

Synonyms

At1g63150 antibody; F16M19.13 antibody; Pentatricopeptide repeat-containing protein At1g63150 antibody

Target Names

At1g63150

Uniprot No.

Q9CAM8

Q&A

Here’s a structured collection of FAQs tailored for academic researchers working with the At1g63150 Antibody, incorporating methodological insights and experimental design considerations:

Advanced Research Questions

How to design a ChIP-Seq experiment for studying At1g63150-DNA interactions?

Workflow:

Step	Parameter	Optimization Tip
Crosslinking	1% formaldehyde, 10 min	Quench with 125 mM glycine
Chromatin shearing	15 cycles (30 sec ON/OFF)	Confirm fragment size (200–500 bp) via agarose gel
Antibody incubation	4 µg antibody per 1 mg chromatin	Include IgG isotype controls

How to analyze contradictory data between genetic and antibody-based localization?

Case Study: If GFP-tagged At1g63150 shows nuclear localization, but antibody staining indicates cytoplasmic presence:
- Validate antibody specificity via knockout controls.
- Check for post-translational modifications affecting epitope accessibility .
- Perform subcellular fractionation + Western blot .

Can single-cell RNA-seq data inform At1g63150 antibody experimental design?

Integration Strategy:
- Use public Arabidopsis single-cell datasets (e.g., Arabidopsis Root Atlas) to identify cell types with high At1g63150 expression.
- Correlate with antibody-based protein detection using spatial transcriptomics .
- Apply computational models predicting antibody binding efficiency based on transcript abundance .

Methodological Challenges & Solutions

Quantifying low-abundance At1g63150 protein variants

Advanced Technique:
- Combine immunoprecipitation (IP) with tandem mass spectrometry (MS/MS).
- Use stable isotope labeling (SILAC) for absolute quantification.
- Reference: Similar workflows were validated for ASK1 phosphorylation studies .

Structural modeling of antibody-antigen interactions

Computational Pipeline:
- Generate homology model of At1g63150 using AlphaFold2.
- Perform molecular docking (e.g., HADDOCK) with antibody Fab fragments .
- Validate critical residues via site-directed mutagenesis.

Data Interpretation Framework

Key Parameters for Cross-Study Comparisons:

Variable	Standardization Method
Antibody Lot	Include internal reference samples in all experiments
Tissue Source	Use ≥3 biological replicates from synchronized plants
Detection Method	Normalize signals to housekeeping proteins and spike-in controls

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At1g63150 Antibody