At1g69290 Antibody
Description
Definition and Target Protein
At1g69290 antibody is an immunological reagent targeting the pentatricopeptide repeat-containing protein At1g69290 in Arabidopsis thaliana. This protein, encoded by the gene At1g69290, belongs to the pentatricopeptide repeat (PPR) superfamily, which is involved in RNA binding, editing, and processing in plant organelles . The protein comprises 658 amino acids and contains conserved PPR motifs critical for its role in mitochondrial or plastid RNA metabolism .
Antigenic Regions
The antibody is generated against synthetic peptides representing specific regions of the protein:
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N-terminus: Amino acids 1–30
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C-terminus: Amino acids 629–658
Antibody Types
| Type | Host Species | Clonality | Applications |
|---|---|---|---|
| Monoclonal (X-P0C7R4) | Mouse | Combination mAbs | ELISA, Western Blot (WB) |
| Polyclonal | Rabbit | Antigen-affinity | WB, Immunoprecipitation (IP) |
Monoclonal antibodies from Abmart are provided as combinations (e.g., X-P0C7R4-N for N-terminus targeting) with ELISA titers of 10,000 and WB detection limits of ~1 ng .
Subcellular Localization
Studies using fluorescent tagging and mass spectrometry confirm that At1g69290 localizes to mitochondria or plastids, consistent with its role in organellar RNA processing .
| Study Model | Localization Prediction | Experimental Confirmation |
|---|---|---|
| Arabidopsis | Mitochondria/Plastid | Fluorescent signal (M/C) |
| Transfected COS-7 | N/A | Mitochondrial targeting |
Biological Role
At1g69290 participates in:
Key Products
| Supplier | Product Code | Type | Price (USD) | Applications |
|---|---|---|---|---|
| Abmart | X-P0C7R4-N | Monoclonal | $599 | ELISA, WB |
| MyBioSource | MBS7150860 | Polyclonal | Undisclosed | WB, ELISA, IP |
Experimental Validation
Applications in Plant Biology
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Functional Studies: Elucidating RNA metabolism mechanisms in plant organelles .
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Diagnostic Tools: Used in Western blotting to validate CRISPR-edited Arabidopsis lines .
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Protein Interaction Mapping: Identifies binding partners via immunoprecipitation .
Challenges and Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Q&A
Based on a comprehensive analysis of available research literature, here is a structured FAQ addressing key technical and methodological aspects of working with At1g69290 antibody in plant molecular biology research. While no direct references to At1g69290 antibody were found in the examined literature, these questions are framed using established antibody development and validation methodologies from related studies.
Advanced Research Questions
How to resolve contradictory ChIP-seq vs Y1H data for At1g69290 binding targets?
Conflict Resolution Workflow:
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Technical audit:
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Verify antibody batch consistency (historical lot data)
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Re-analyze raw sequencing data with updated genome annotation
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Biological context analysis:
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Compare growth conditions (light/temp differences between studies)
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Test dimerization partners through co-IP/MS
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Functional validation:
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CRISPR-Cas9 editing of disputed binding sites
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Luciferase reporter assays with mutated cis-elements
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Approach adapted from TF target validation in and antibody characterization in
How to establish species cross-reactivity while maintaining specificity?
Develop a decision matrix:
| Test Species | Expected Cross-Reactivity | Validation Assay |
|---|---|---|
| Arabidopsis (Col-0) | 100% (positive control) | Western blot |
| Arabidopsis (Ler) | ≥90% | ELISA |
| Brassica napus | 50-70% | Immunofluorescence |
| Oryza sativa | ≤10% | SPR kinetics |
Design principle from multi-species validation in and
What statistical approaches handle batch effects in long-term studies?
Implement mixed-effects model:
Where:
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: Antibody lot fixed effect
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: Experimental batch random effect
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: Measurement error
Critical validation steps:
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ANOVA with Tukey post-hoc for lot comparisons
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PCA of raw fluorescence intensities
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Standard curve parallelism testing
