At1g79080 Antibody
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Product Specs
Buffer
Preservative: 0.03% Proclin 300
Composition: 50% Glycerol, 0.01M PBS, pH 7.4
Composition: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
At1g79080 antibody; YUP8H12R.30 antibody; Pentatricopeptide repeat-containing protein At1g79080 antibody; chloroplastic antibody
Target Names
At1g79080
Uniprot No.
Target Background
Database Links
Protein Families
PPR family, P subfamily
Subcellular Location
Plastid, chloroplast.
Q&A
Here’s a structured FAQ collection for researchers working with the At1g79080 Antibody in Arabidopsis thaliana studies, synthesized from technical literature and methodological best practices:
What experimental controls are critical for At1g79080 localization studies?
Advanced Considerations:
How to resolve contradictory data in At1g79080 interaction studies?
Troubleshooting Framework:
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Assay Discrepancy: Compare results across methods (e.g., yeast two-hybrid vs. co-IP).
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Epitope Accessibility: Use truncated protein variants to map interaction domains .
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Batch Variation: Employ QC metrics like mass accuracy (<25 ppm) and glycan profiling .
What computational tools predict At1g79080 binding partners?
Advanced Workflow:
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Use library-on-library active learning models to prioritize antigen-antibody pairs for testing .
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Validate predictions with differential hydrogen-deuterium exchange (HDX-MS) to map conformational epitopes .
How to design a kinetic study for At1g79080 expression?
QC Parameters:
| Metric | Target | Method |
|---|---|---|
| Mass Accuracy | <25 ppm | ESI-TOF MS |
| Glycan Variance | ≤5% | HPLC-Poroshell C8 |
| Cross-Reactivity | 0% vs. At4g21170 | Peptide microarray |
How to map conformational epitopes for At1g79080?
Structural Approach:
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