Customize At2g01360 Antibody

Description

Introduction to At2g01360 Antibody

The At2g01360 antibody is a polyclonal antibody developed against the Arabidopsis thaliana (Mouse-ear cress) protein encoded by the At2g01360 gene. This antibody is primarily utilized in plant molecular biology research to study the expression, localization, and functional roles of the At2g01360 protein, which remains under investigation for its specific biological pathways .

Target Protein Characteristics

The At2g01360 gene encodes a protein of unknown function, though homology studies suggest potential involvement in stress response or metabolic regulation. Its UniProt entry (Q9ZU31) classifies it as a member of a conserved protein family with structural motifs indicative of enzymatic or regulatory activity .

Functional Insights

Antibodies against Arabidopsis proteins often enable discoveries in gene silencing, pathogen response, and developmental biology. For instance, antibodies targeting ARG2 (a related enzyme) restored T-cell proliferation in leukemia models by blocking immunosuppressive pathways . Such approaches could guide future work on At2g01360’s role in plant immunity or stress adaptation.

Specificity Assurance

Knockout Validation: Recommended to compare wild-type and At2g01360 knockout Arabidopsis lines to rule off-target binding .
Epitope Mapping: Linear epitopes are confirmed via peptide arrays or competitive ELISA, as demonstrated in monoclonal antibody development workflows .

Cross-Reactivity

No cross-reactivity with human or murine proteins has been reported, though plant-specific antibodies often require species-specific validation .

Comparative Analysis with Related Antibodies

Antibody	Target	Applications	Specificity
At2g01360 Antibody	At2g01360 (Q9ZU31)	WB, IF, ELISA	Arabidopsis thaliana
ARG2 Inhibitor (C0021158)	Human ARG2	T-cell proliferation	Human
Anti-A2AR (TB206-001)	Adenosine A2A receptor	Tumor suppression	Human/murine

Future Directions

Further research should prioritize:

Functional Characterization: Linking At2g01360 to specific biochemical pathways using CRISPR-edited Arabidopsis lines.
Therapeutic Potential: Exploring plant-derived antibodies for agricultural biotechnology, inspired by tumor-suppressing antibody mechanisms .

Product Specs

Buffer

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

14-16 week lead time (made-to-order)

Synonyms

At2g01360 antibody; F10A8.24 antibody; Pentatricopeptide repeat-containing protein At2g01360 antibody

Target Names

At2g01360

Uniprot No.

Q9ZU31

Q&A

FAQs for At2g01360 Antibody in Academic Research

Advanced Research Questions

How to resolve discrepancies in At2g01360 expression data across studies?
- Methodological harmonization:
  - Standardize tissue harvesting times (e.g., circadian rhythm impacts).
  - Use spike-in controls (e.g., exogenous proteins) to normalize quantification in western blots .
- Data contradiction analysis: Compare antibody batches (e.g., lot-specific variability) and epitope accessibility under different fixation methods .
What computational tools can predict At2g01360 epitopes for antibody validation?
- Energy-based optimization: Apply residue-level energy decomposition (e.g., Rosetta binding energy models) to map antibody-antigen interaction hotspots .
- Structural alignment: Use AlphaFold-predicted At2g01360 structures to assess epitope conservation across Arabidopsis ecotypes .
How to design a study investigating At2g01360’s role in stress response pathways?
- Multi-omics integration:
  - Pair antibody-based protein quantification with RNA-seq data from abiotic stress treatments.
  - Employ pathway enrichment analysis (e.g., MapMan) to link At2g01360 to specific stress modules .
- Table: Key parameters for stress-response experiments
  
  Stress Type Treatment Duration Tissue Sampled Antibody Dilution
  Drought 0h, 6h, 24h Roots 1:1,000
  Salt 12h, 48h Leaves 1:2,000

Stress Type	Treatment Duration	Tissue Sampled	Antibody Dilution
Drought	0h, 6h, 24h	Roots	1:1,000
Salt	12h, 48h	Leaves	1:2,000

Methodological Considerations

What are the limitations of At2g01360 Antibody in detecting post-translational modifications (PTMs)?
- Phosphorylation/Oxidation: Use 2D gel electrophoresis followed by western blotting to separate modified isoforms.
- Alternative approach: Generate custom PTM-specific antibodies or pair with pan-antibodies in multiplex assays .
How to optimize ChIP-seq protocols for At2g01360 chromatin interaction studies?
- Crosslinking: Test formaldehyde vs. dual crosslinkers (e.g., DSG) for nuclear membrane integrity.
- Antibody validation: Include a peptide competition assay (pre-incubate antibody with At2g01360 peptide) to confirm signal specificity .

Data Interpretation Guidelines

Quantitative thresholds: Define signal-to-noise ratios (e.g., ≥3:1 for western blot bands) to minimize false positives .
Reproducibility: Replicate experiments across independent Arabidopsis growth batches to account for environmental variability .

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At2g01360 Antibody