Customize At2g02150 Antibody

Description

Molecular Identity and Function

At2g02150 (UniProt: P0C894) is a nuclear-encoded mitochondrial protein belonging to the PPR family, characterized by tandem repeats involved in RNA binding and processing . The protein is essential for:

Mitochondrial RNA splicing: Specifically, trans-splicing of nad2 intron 2, critical for Complex I (CI) biogenesis .
Embryogenesis: Homozygous emb2794 mutants exhibit arrested embryo development at the globular stage, linked to defective CI assembly .

Genetic and Phenotypic Analysis

Parameter	Observation	Source
Knockout phenotype	Embryonic lethality; CI activity undetectable
RNA splicing defects	Impaired nad2 intron 2 trans-splicing
Rescue experiments	Complementation with 35S::At2g02150::GFP restores wild-type phenotype

Antibody Applications

Localization studies: Used to confirm mitochondrial localization of EMB2794 .
Functional assays: Validates protein expression in transgenic rescue lines .

Research Implications

Mitochondrial biogenesis: At2g02150 is a key player in stabilizing mitochondrial RNA intermediates, with defects leading to CI dysfunction .
Agricultural biotechnology: Understanding embryogenesis defects may inform seed viability studies in crops .

Product Specs

Buffer

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

14-16 week lead time (made-to-order)

Synonyms

At2g02150 antibody; F5O4.8 antibody; Putative pentatricopeptide repeat-containing protein At2g02150 antibody

Target Names

At2g02150

Uniprot No.

P0C894

Q&A

The At2g02150 antibody targets a protein encoded by the Arabidopsis thaliana gene AT2G02150, though specific characterization data for this antibody isn't directly available in published literature. Below are research-focused FAQs addressing common methodological challenges in antibody validation and application, synthesized from broader antibody development studies .

Advanced Research Challenges

How to resolve contradictory western blot results between laboratories using the same antibody?

Conduct systematic protocol alignment:
- Compare lysis buffers (SDS vs. RIPA extraction impacts epitope availability)
- Standardize protein loading amounts (10-50μg range validation recommended)
- Validate transfer efficiency using prestained markers across PVDF/nitrocellulose membranes

What computational methods enhance antibody validation for low-abundance targets?

Combine:
- Machine learning prediction: Use AlphaFold2 models to verify epitope accessibility in 3D protein structure
- Digital PCR: Quantify target mRNA levels to establish protein expression baselines
- Single-cell proteomics: Map antibody signal distribution across cell populations

Data Interpretation Guidance

How to distinguish true signal from background in IHC experiments?

Apply quantitative threshold analysis:

Parameter	Calculation Method	Validation Benchmark
Signal-to-noise ratio	(Mean test signal - negative control)/SD controls	≥3:1 required
Spatial consistency	Correlation with transcriptional reporter lines	R² ≥0.7
Dose response	Serial dilution curve linearity	R² ≥0.95 across 3 logs

Reproducibility Framework

What metadata should accompany publications using this antibody?

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At2g02150 Antibody