PPP3R2 Human
Description
Overview of PPP3R2
PPP3R2, also known as Calcineurin subunit B type 2, is a regulatory component of the calcineurin protein phosphatase complex. It functions as a calcium sensor, enabling calcineurin’s activation in response to intracellular calcium fluctuations . Key characteristics include:
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Expression: Primarily in testis and sperm, with unique localization shifts during sperm maturation .
Physical Properties
| Property | Detail | Source |
|---|---|---|
| Expression System | Escherichia coli | |
| Purity | >95% (SDS-PAGE verified) | |
| Stability | Requires carrier protein (HSA/BSA) for long-term storage |
Functional Role in Calcium Signaling
PPP3R2 binds to catalytic subunits (PPP3CA, PPP3CB, PPP3CC) to form the calcineurin holoenzyme, which dephosphorylates substrates like NFAT transcription factors (NFATC1, NFATC3) . Key mechanisms include:
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Calcium sensitivity: Confers Ca²⁺/calmodulin-dependent activation .
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Sperm maturation: Essential for structural integrity and motility in murine sperm; knockout models exhibit infertility due to flagellar defects .
Knockout Mouse Phenotype
| Parameter | Observation | Source |
|---|---|---|
| Fertility | Complete male infertility | |
| Sperm Motility | Severely reduced (<10% of wild type) | |
| Flagellar Structure | Disrupted midpiece-principal piece transition |
Clinical Relevance in Male Infertility
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PPP3R2 deficiency correlates with teratozoospermia (abnormal sperm morphology) .
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No direct association with asthenozoospermia (reduced sperm motility) in humans, unlike its catalytic partner PPP3CC .
Interaction Network
PPP3R2 partners with proteins critical for immune and neuronal signaling :
| Interactor | Role | Score (STRING) |
|---|---|---|
| PPP3CA | Catalytic subunit | 0.993 |
| NFATC1 | Transcriptional regulator of IL-2 | 0.948 |
| SSH3 | Actin depolymerization regulator | 0.906 |
Applications in Research
Product Specs
Calcineurin subunit B type 2, Calcineurin B-like protein, CBLP, Calcineurin BII, CNBII, PPP3R1-like, Protein phosphatase 2B regulatory subunit 2, Protein phosphatase 3 regulatory subunit B beta isoform, PPP3R2, PPP3RL.
MGSSHHHHHH SSGLVPRGSH MSTMGNEASY PAEMCSHFDN DEIKRLGRRF KKLDLDKSGS LSVEEFMSLP ELRHNPLVRR VIDVFDTDGD GEVDFKEFIL GTSQFSVKGD EEQKLRFAFS IYDMDKDGYI SNGELFQVLK MMVGNNLTDW QLQQLVDKTI IILDKDGDGK ISFEEFSAVV RDLEIHKKLV LIV.
Product Science Overview
Protein Phosphatase 3, also known as Calcineurin, is a calcium/calmodulin-dependent serine/threonine protein phosphatase. It plays a crucial role in various cellular processes, including T-cell activation, muscle development, and neuronal signaling. The regulatory subunit, Beta Isozyme (PPP3R2), is one of the key components that modulate the activity of Calcineurin.
The Beta Isozyme of Protein Phosphatase 3 Regulatory Subunit (PPP3R2) is a protein coding gene. It is involved in the regulation of the catalytic subunit of Calcineurin (PPP3CB). The regulatory subunit ensures the proper functioning of the phosphatase by binding to the catalytic subunit and modulating its activity in response to calcium signals .
The human recombinant form of PPP3R2 is typically produced in E. coli. The recombinant protein is fused with a His tag at the N-terminus, which facilitates its purification through chromatographic techniques. The resulting product is a single, non-glycosylated polypeptide chain containing 193 amino acids and having a molecular mass of approximately 22 kDa .
PPP3R2 is essential for the regulation of Calcineurin activity. Calcineurin is involved in the dephosphorylation of various substrates, including the nuclear factor of activated T-cells (NFAT), which is crucial for T-cell activation and immune response. Dysregulation of Calcineurin activity has been implicated in several diseases, including autoimmune disorders and neurodegenerative diseases .
The human recombinant form of PPP3R2 is widely used in research to study the regulatory mechanisms of Calcineurin. It is also utilized in drug discovery efforts aimed at modulating Calcineurin activity for therapeutic purposes. Understanding the interaction between PPP3R2 and the catalytic subunit of Calcineurin can provide insights into the development of novel treatments for diseases associated with Calcineurin dysregulation .
