At1g20300 Antibody

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Description

Analysis of Available Data

The search results focus on:

  • General antibody structure/function ( )

  • Human therapeutic antibodies ( )

  • Antibodies targeting angiotensin II type 1 receptor (AT1R) in COVID-19 and autoimmune diseases ( )

  • Methodological challenges in AT1 receptor antibody validation ( )

No references to "At1g20300" or Arabidopsis-related antibodies were found in any source.

Nomenclature Considerations

AspectDetail
Gene ID FormatArabidopsis genes use "At[chromosome]g[position]" format (e.g., At1g20300)
Antibody TargetsTypically use protein names (e.g., AT1R) rather than gene identifiers
Species SpecificityAll examined antibodies target human/mammalian proteins ([1-9])

Technical Limitations

  • Commercial antibodies against plant proteins are less common than human/mammalian targets

  • No publications cite At1g20300 antibodies in PubMed Central ( ), Frontiers ( ), or other indexed journals

Recommendations for Further Investigation

  1. Verify Compound Name

    • Confirm if "At1g20300" refers to:

      • Arabidopsis gene AT1G20300 (annotated as Pentatricopeptide repeat protein)

      • Potential typo for AT1R (angiotensin receptor antibodies discussed in )

  2. Alternative Research Pathways

    • Request protein sequence from Arabidopsis databases (TAIR, UniProt)

    • Develop custom polyclonal antibodies through peptide immunization

    • Explore homology with known antibody targets using BLAST alignment

Related Antibody Research Context

While At1g20300-specific data is unavailable, these established antibody characterization methods could apply:

Validation Protocols ( )

StepRequirement
Specificity TestingKnockout controls (e.g., AT1A -/- mice in )
Western BlotExpected band at predicted molecular weight
Functional AssaysReceptor activation/inhibition measurements

Production Considerations ( )

FeatureVivopureX™ Example
Species EngineeringMouse IgG Fc optimization
Effector FunctionTailored Fc receptor binding
Batch ConsistencyRecombinant production

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
At1g20300 antibody; F14O10.10 antibody; Pentatricopeptide repeat-containing protein At1g20300 antibody; mitochondrial antibody
Target Names
At1g20300
Uniprot No.

Target Background

Database Links

KEGG: ath:AT1G20300

STRING: 3702.AT1G20300.1

UniGene: At.26508

Protein Families
PPR family, P subfamily
Subcellular Location
Mitochondrion.

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