The search results focus on:
Antibodies targeting angiotensin II type 1 receptor (AT1R) in COVID-19 and autoimmune diseases ( )
Methodological challenges in AT1 receptor antibody validation ( )
No references to "At1g20300" or Arabidopsis-related antibodies were found in any source.
| Aspect | Detail |
|---|---|
| Gene ID Format | Arabidopsis genes use "At[chromosome]g[position]" format (e.g., At1g20300) |
| Antibody Targets | Typically use protein names (e.g., AT1R) rather than gene identifiers |
| Species Specificity | All examined antibodies target human/mammalian proteins ([1-9]) |
Commercial antibodies against plant proteins are less common than human/mammalian targets
No publications cite At1g20300 antibodies in PubMed Central ( ), Frontiers ( ), or other indexed journals
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Alternative Research Pathways
Request protein sequence from Arabidopsis databases (TAIR, UniProt)
Develop custom polyclonal antibodies through peptide immunization
Explore homology with known antibody targets using BLAST alignment
While At1g20300-specific data is unavailable, these established antibody characterization methods could apply:
| Step | Requirement |
|---|---|
| Specificity Testing | Knockout controls (e.g., AT1A -/- mice in ) |
| Western Blot | Expected band at predicted molecular weight |
| Functional Assays | Receptor activation/inhibition measurements |
| Feature | VivopureX™ Example |
|---|---|
| Species Engineering | Mouse IgG Fc optimization |
| Effector Function | Tailored Fc receptor binding |
| Batch Consistency | Recombinant production |