At1g22960 Antibody
Description
Identification and Target Specificity
The At1g22960 antibody targets the pentatricopeptide repeat (PPR) superfamily protein encoded by the At1g22960 gene in Arabidopsis thaliana. This mitochondrial protein is critical for RNA editing and post-transcriptional regulation in plant organelles .
| Parameter | Details |
|---|---|
| Gene Names | At1g22960, F19G10.9, F19G10_9 |
| Protein Family | Pentatricopeptide repeat (PPR) superfamily |
| Host Species | Rabbit |
| Reactivity | Arabidopsis thaliana (Mouse-ear cress) |
| Applications | ELISA, Western Blot (WB) |
| Purification Method | Antigen-affinity chromatography |
| Isotype | IgG |
Molecular and Functional Insights
The PPR protein family is characterized by tandem repeats of 35-amino-acid motifs that facilitate sequence-specific RNA binding. The At1g22960 protein localizes to mitochondria, where it likely participates in:
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RNA processing and stabilization
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Organellar gene expression
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Plant developmental regulation
This antibody enables detection of the endogenous protein in Arabidopsis tissues, aiding studies on mitochondrial RNA metabolism .
Experimental Validation
The antibody’s utility has been confirmed through:
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Western Blot: Specific detection of the ~70 kDa At1g22960 protein in mitochondrial extracts.
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ELISA: Quantification of protein expression under stress or developmental conditions.
No cross-reactivity with non-target proteins has been reported, ensuring high specificity for Arabidopsis samples .
Functional Genomics
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Investigating mitochondrial RNA editing defects in Arabidopsis mutants.
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Characterizing PPR protein interactions with organellar transcripts.
Developmental Studies
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Mapping tissue-specific expression patterns (e.g., roots, leaves).
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Assessing protein abundance during stress responses (e.g., drought, pathogens).
Product Specs
Target Background
Q&A
FAQs for Researchers on AT1G22960 Antibody
Advanced Research Questions
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How to resolve discrepancies between predicted and observed AT1G22960 localization?
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Stepwise approach:
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Key metric: ≥90% colocalization with mitochondrial markers (e.g., COXII) required for validation.
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What computational strategies predict AT1G22960 antibody-epitope interactions?
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Framework:
Method Application Validation Requirement Biophysical modeling Energy landscape prediction SPR/ITC binding assays Phage display libraries Epitope mapping Deep mutational scanning -
Reference: Machine learning models trained on multi-ligand selection data can disentangle binding modes for mitochondrial PPR proteins .
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Methodological Challenges
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Designing antibodies to distinguish AT1G22960 from paralogs (e.g., AT1G22950):
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Interpreting negative results in mitochondrial interaction studies:
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Troubleshooting table:
Issue Diagnostic Test Mitigation Antibody penetration failure IgG control in immunogold EM Use freeze-thaw permeabilization Transient interactions Crosslinking-MS (e.g., DSG) Increase temporal resolution of sampling
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Data Generation & Curation
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Standardizing validation metrics for AT1G22960 functional studies:
