Function
This antibody targets the phosphoenolpyruvate/phosphate translocator (PPT1), a protein responsible for transporting phosphoenolpyruvate (PEP), 2-phosphoglycerate, 3-phosphoglycerate, and dihydroxyacetone phosphate. PPT1 imports PEP into the chloroplast stroma, a crucial substrate for the shikimate pathway. This pathway is essential for the biosynthesis of aromatic amino acids and various secondary metabolites. PPT1 is also required for proper leaf mesophyll cell development and the expression of chlorophyll a/b binding protein 3 (CAB3).
Gene References Into Functions
Further research highlights the importance of PPT1. For example, studies screening for genetic suppressors of *ros1* identified two mutant alleles in the *Arabidopsis thaliana* CHLOROPHYLL A/B BINDING PROTEIN UNDEREXPRESSED1 (*CUE1*) gene, which is related to PPT1 function. [PMID: 19515789](https://www.ncbi.nlm.nih.gov/pubmed/19515789)
Protein Families
TPT transporter family, PPT (TC 2.A.7.9) subfamily
Subcellular Location
Plastid, chloroplast membrane; Multi-pass membrane protein.
Tissue Specificity
Expressed in root columella, lateral root cap and root vasculature tissue. In leaves, highly expressed in xylem parenchyma cells. In flowers, expressed in sepals, petals, filaments of the stamens, anthers and stigma.