PPWD1 Antibody
Description
Overview of PPWD1 Antibody
PPWD1 (Peptidylprolyl Isomerase Domain and WD Repeat Containing 1) antibodies are polyclonal reagents generated in rabbits, targeting specific regions of the PPWD1 protein. These antibodies are widely used to investigate PPWD1's function in cellular processes such as spliceosome activity and metabolic regulation .
Validation and Performance
PPWD1 antibodies have been validated across diverse samples:
Table 1: Validated Tissues/Cell Lines
| Sample Type | Detection Confirmed |
|---|---|
| Mouse tissues | Brain, kidney, liver, testis |
| Human cell lines | HeLa, HEK-293, Jurkat |
| Other species | Rat, dog, zebrafish |
Recommended dilutions for WB range from 1:500 to 1:1000, though optimization is advised .
Role in Postmenopausal Osteoporosis (PMO)
A 2024 study identified PPWD1 as a biomarker for PMO through gene co-expression network analysis :
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Expression Correlation: PPWD1 levels in blood monocytes inversely correlated with bone mineral density (BMD) in postmenopausal women.
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Functional Link: Enrichment analysis tied PPWD1 to fatty acid metabolism pathways, suggesting its role in bone density regulation .
Molecular Mechanisms
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Spliceosome Function: PPWD1 is part of the spliceosome C complex, implicating it in pre-mRNA processing .
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Energy Metabolism: PPWD1 may influence mitochondrial oxidative phosphorylation, impacting cellular energy balance .
Comparative Antibody Performance
Product Specs
Target Background
- The crystal structure of human PPWD1 has been studied. PMID: 18397323
