PRDM7 Antibody
Description
PRDM7 Antibody: Definition and Functional Context
PRDM7 is a zinc finger protein with a PR/SET domain that catalyzes histone H3 lysine 4 trimethylation (H3K4me3), a hallmark of active chromatin . The antibody targets PRDM7 for applications including:
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Western blot (WB)
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Immunohistochemistry (IHC)
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Immunofluorescence (IF/ICC)
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Enzyme-linked immunosorbent assay (ELISA)
Key roles of PRDM7 in research include studying:
Catalytic Activity of PRDM7
PRDM7 exhibits distinct enzymatic properties compared to its paralog PRDM9 :
| Parameter | PRDM7 Wild-Type | PRDM7 S357Y Mutant | PRDM9 Wild-Type |
|---|---|---|---|
| Primary Substrate | H3K4me2 → H3K4me3 | H3K4me2 → H3K4me3 | H3K4me2 → H3K4me3 |
| Secondary Activity | None | H3K36 methylation | H3K36 methylation |
| AdoMet K<sub>m</sub> | 1,000 μM | 250 μM | 50 μM |
The S357Y mutation restores PRDM9-like dual substrate specificity, linking evolutionary divergence to functional adaptation .
Role in Disease
PRDM7 is implicated in cancer through:
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Genomic instability: Loss of PRDM7 disrupts DNA repair mechanisms, promoting oncogenic mutations .
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Histone code regulation: Altered H3K4me3 levels correlate with tumor progression in lung adenocarcinoma and leukemia .
Validation Data and Technical Notes
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research indicates that a single serine to tyrosine mutation at residue 357 (S357Y) restores the substrate specificities and catalytic activities of PRDM7 to resemble its evolutionary predecessor, including the ability to effectively methylate H3K36. PMID: 27129774
- Evolutionary analysis suggests that human PRDM7 and PRDM9 genes, which are closely related paralogs corresponding to a single mouse gene Prdm9, originated from a recent gene duplication event that occurred after the divergence of human and mouse ancestors. PMID: 18231586
