Phospho-CHEK2-S516 pAb Antibodies
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Phospho-CHEK2-S516 pAb

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog No: bt-239770

Product Name Phospho-CHEK2-S516 pAb
Application WB
Application Key Western blotting
Calculated Mw 15-38kDa/50-65kDa
Cellular Location Nucleus,Nucleus,PML body,nucleoplasm
Host Species Rabbit
Immunogen A phospho specific peptide corresponding to residues surrounding S516 of human CHEK2
Isotype IgG
Observed Mw 61kDa
Product Background In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Positive Samples A-549
Purification Method Affinity purification
Recommended Dilution WB
Sequence Email for sequence
Species Reactivity Human
Storage Buffer Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.