Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (ECD, aa 1-1297, His Tag) Antigens The extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012)( AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus. The recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012) comprises 1289a.a and has a predicted molecular weight of 142.2 kDa. It migrates as an approximately 157 kDa band in SDS-PAGE under reducing conditions.
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Formulation: The recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012) comprises 1289a.a and has a predicted molecular weight of 142.2 kDa. It migrates as an approximately 157 kDa band in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (ECD, aa 1-1297, His Tag)

Formulation: Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein was lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol.1.

Source: Baculovirus-Insect Cells

Product Name Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (ECD, aa 1-1297, His Tag)
Product Type Antigen
Source Baculovirus-Insect Cells
Formulation Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein was lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol.1.
Molecular Weight The recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012) comprises 1289a.a and has a predicted molecular weight of 142.2 kDa. It migrates as an approximately 157 kDa band in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 85% as determined by SDS-PAGE
Description The extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012)( AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus.
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met1-Trp1297
Product Background The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
Memo S Protein, MERS-CoV
Terminal Tyr 18
Bioactivity Measured by its binding ability in a functional ELISA.1.Immobilized Sh(2C-EMC)(1-1297)(Cat 40069-V08B) at 10 ug/mL (100 ul/well) can bind biotinylated DPP4,The EC50 of of biotinylated DPP4 is 0.02-0.04 ug/mL.2.Immobilized Sh(2C-EMC)(1-1297)(Cat 40069-V08B) at 10 ug/mL (100 ul/well) can bind biotinylated Fc-DPP4,The EC50 of can biotinylated Fc-DPP4 is 0.01-0.02 ug/mL.