Recombinant Zika virus (ZIKV) (strain Zika SPH2015) E / Envelope protein (aa 291-696, His Tag) Antigens The Zika virus (strain Zika SPH2015) E (ALU33341.1, with mutation Thr 356 Ala, Gln 357 Gly, Trp 381 Arg, Leu 387 Arg) (Ile291-Thr696) was expressed with a polyhistidine tag at the C-terminus. The recombinant Zika virus (strain Zika SPH2015) E consists of 417a.a and predicts a molecular weight of 45.6 kDa.
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Formulation: The recombinant Zika virus (strain Zika SPH2015) E consists of 417a.a and predicts a molecular weight of 45.6 kDa.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Recombinant Zika virus (ZIKV) (strain Zika SPH2015) E / Envelope protein (aa 291-696, His Tag)

Formulation: Recombinant Zika virus (ZIKV) (strain Zika SPH2015) E / Envelope Protein was lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 8.0, 10 % glycerol.1.

Source: Baculovirus-Insect Cells

Product Name Recombinant Zika virus (ZIKV) (strain Zika SPH2015) E / Envelope protein (aa 291-696, His Tag)
Product Type Antigen
Source Baculovirus-Insect Cells
Formulation Recombinant Zika virus (ZIKV) (strain Zika SPH2015) E / Envelope Protein was lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 8.0, 10 % glycerol.1.
Molecular Weight The recombinant Zika virus (strain Zika SPH2015) E consists of 417a.a and predicts a molecular weight of 45.6 kDa.
Endotoxin < 1.0 EU per μg protein as determined by the LAL method.
Purity Greater than 90% as determined by SDS-PAGE.
Description The Zika virus (strain Zika SPH2015) E (ALU33341.1, with mutation Thr 356 Ala, Gln 357 Gly, Trp 381 Arg, Leu 387 Arg) (Ile291-Thr696) was expressed with a polyhistidine tag at the C-terminus.
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Ile291-Thr696
Product Background Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis.The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Terminal Ile 291
Bioactivity Please contact us for detailed information