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Recombinant Influenza A H7N9 (A/Shanghai/4664T/2013) Hemagglutinin / HA Protein (His Tag) Antigens The Influenza A virus (A/Shanghai/4664T/2013(H7N9)) hemagglutinin (AGI60292.1) (Met1-Val524) was expressed with a C-terminal polyhistidine tag. The recombinant hemagglutinin of Influenza A virus (A/Shanghai/4664T/2013(H7N9)) comprises 517a.a and has a predicted molecular weight of 57.6 kDa. The apparent molecular weight of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions.
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Formulation: The recombinant hemagglutinin of Influenza A virus (A/Shanghai/4664T/2013(H7N9)) comprises 517a.a and has a predicted molecular weight of 57.6 kDa. The apparent molecular weight of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Recombinant Influenza A H7N9 (A/Shanghai/4664T/2013) Hemagglutinin / HA Protein (His Tag)

Formulation: Recombinant Influenza A H7N9 (A/Shanghai/4664T/2013) Hemagglutinin / HA Protein was lyophilized from sterile 20 mM Tris, 500 mM Nacl, 10 % glycerol, pH 8.0.1.

Source: Baculovirus-Insect Cells

Product Name Recombinant Influenza A H7N9 (A/Shanghai/4664T/2013) Hemagglutinin / HA Protein (His Tag)
Product Type Antigen
Source Baculovirus-Insect Cells
Formulation Recombinant Influenza A H7N9 (A/Shanghai/4664T/2013) Hemagglutinin / HA Protein was lyophilized from sterile 20 mM Tris, 500 mM Nacl, 10 % glycerol, pH 8.0.1.
Molecular Weight The recombinant hemagglutinin of Influenza A virus (A/Shanghai/4664T/2013(H7N9)) comprises 517a.a and has a predicted molecular weight of 57.6 kDa. The apparent molecular weight of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 95% as determined by SDS-PAGE
Description The Influenza A virus (A/Shanghai/4664T/2013(H7N9)) hemagglutinin (AGI60292.1) (Met1-Val524) was expressed with a C-terminal polyhistidine tag.
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met1-Val524
Product Background The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Terminal Asp 19
Bioactivity 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.6 ug/mL for 1% GRBC.