Recombinant Influenza A H5N1 (A/barnswallow/HongKong/D10-1161/2010) Hemagglutinin Protein (HA1+HA2) (His Tag) Antigens The Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) hemagglutinin (AGC13463.1) (Met1-Gln531) with cleavage site mutated (RRRRK →TET, HA1+HA2, uncleaved) was expressed with a C-terminal polyhistidine tag. The recombinant hemagglutinin of Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) comprises 524a.a and has a predicted molecular weight of 59.8 kDa. The apparent molecular weight of the protein is approximately 58.3 kDa in SDS-PAGE under reducing conditions.
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Formulation: The recombinant hemagglutinin of Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) comprises 524a.a and has a predicted molecular weight of 59.8 kDa. The apparent molecular weight of the protein is approximately 58.3 kDa in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Recombinant Influenza A H5N1 (A/barnswallow/HongKong/D10-1161/2010) Hemagglutinin Protein (HA1+HA2) (His Tag)

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog Number: BT-224400

Molecular Weight: The recombinant hemagglutinin of Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) comprises 524a.a and has a predicted molecular weight of 59.8 kDa. The apparent molecular weight of the protein is approximately 58.3 kDa in SDS-PAGE under reducing conditions.

Formulation: Recombinant Influenza A H5N1 (A/barnswallow/HongKong/D10-1161/2010) Hemagglutinin Protein was lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.1.

Source: Baculovirus-Insect Cells

Product Name Recombinant Influenza A H5N1 (A/barnswallow/HongKong/D10-1161/2010) Hemagglutinin Protein (HA1+HA2) (His Tag)
Molecular Weight The recombinant hemagglutinin of Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) comprises 524a.a and has a predicted molecular weight of 59.8 kDa. The apparent molecular weight of the protein is approximately 58.3 kDa in SDS-PAGE under reducing conditions.
AA Sequence Met1-Gln531
Bioactivity 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 2-8 ng/mL for 1% GRBC.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Recombinant Influenza A H5N1 (A/barnswallow/HongKong/D10-1161/2010) Hemagglutinin Protein was lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.1.
Description The Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) hemagglutinin (AGC13463.1) (Met1-Gln531) with cleavage site mutated (RRRRK →TET, HA1+HA2, uncleaved) was expressed with a C-terminal polyhistidine tag.
Product Background The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Product Type Antigen
Purity Greater than 95% as determined by SDS-PAGE
Source Baculovirus-Insect Cells
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Terminal Asp 17
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Last Modified Aug 20 2021