Recombinant Influenza A H1N1 (A/Puerto Rico/8/34) Hemagglutinin / HA Protein (His Tag)
Antigens
The extracellular domain (Met 1-Gln 528) of the influenza A hemagglutinin (A/Puerto Rico/8/34 (H1N1)) (ABD77675.1), was expressed, fused with a C-terminal polyhistidine tag.
The secreted recombinant influenza A H1N1 (A/Puerto Rico/8/1934 (H1N1)) HA comprises 522a.a and has a predicted molecular weight of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
Formulation:
The secreted recombinant influenza A H1N1 (A/Puerto Rico/8/1934 (H1N1)) HA comprises 522a.a and has a predicted molecular weight of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
Source:
HEK293 Cells
Usage:
Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Recombinant Influenza A H1N1 (A/Puerto Rico/8/34) Hemagglutinin / HA Protein (His Tag)
The product is for non-human research only. Not for therapeutic or veterinary use.
Catalog Number: BT-224561
Molecular Weight: The secreted recombinant influenza A H1N1 (A/Puerto Rico/8/1934 (H1N1)) HA comprises 522a.a and has a predicted molecular weight of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
Formulation: Recombinant Influenza A H1N1 (A/Puerto Rico/8/34) Hemagglutinin / HA Protein was lyophilized from sterile PBS, pH 7.41.
Source: HEK293 Cells
| Product Name | Recombinant Influenza A H1N1 (A/Puerto Rico/8/34) Hemagglutinin / HA Protein (His Tag) |
|---|---|
| Molecular Weight | The secreted recombinant influenza A H1N1 (A/Puerto Rico/8/1934 (H1N1)) HA comprises 522a.a and has a predicted molecular weight of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions. |
| AA Sequence | Met 1-Gln 528 |
| Bioactivity | Please contact us for detailed information |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Formulation | Recombinant Influenza A H1N1 (A/Puerto Rico/8/34) Hemagglutinin / HA Protein was lyophilized from sterile PBS, pH 7.41. |
| Description | The extracellular domain (Met 1-Gln 528) of the influenza A hemagglutinin (A/Puerto Rico/8/34 (H1N1)) (ABD77675.1), was expressed, fused with a C-terminal polyhistidine tag. |
| Product Background | The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
| Product Type | Antigen |
| Purity | Greater than 90% as determined by SDS-PAGE |
| Source | HEK293 Cells |
| Storage | Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Terminal | Asp 18 |
| Usage | Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use. |
| Last Modified | Aug 20 2021 |