2'-Deoxycytidine-5'-triphosphate
Catalog Number: BT-255745
CAS Number: 2056-98-6
Molecular Formula: C₉H₁₆N₃O₁₃P₃
Molecular Weight: 463.13 g/mol
The product is for non-human research only. Not for therapeutic or veterinary use.
Price:
Product Introduction
Description
2'-Deoxycytidine-5'-triphosphate (dCTP) is a nucleotide that plays a crucial role in DNA synthesis and repair. It is a building block of DNA and is essential for the maintenance of genetic information. dCTP is synthesized in cells through a complex biochemical pathway involving several enzymes. It is also available commercially for use in various research applications.
Properties
CAS Number
2056-98-6
Product Name
2'-Deoxycytidine-5'-triphosphate
IUPAC Name
[[(2R,3S,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
Molecular Formula
C₉H₁₆N₃O₁₃P₃
Molecular Weight
463.13 g/mol
InChI
InChI=1S/C9H16N3O13P3/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17)/t5-,6+,8+/m0/s1
InChI Key
RGWHQCVHVJXOKC-SHYZEUOFSA-N
SMILES
C1C(C(OC1N2C=CC(=NC2=O)N)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O
Synonyms
2'-deoxycytidine 5'-triphosphate
2'-deoxycytidine 5'-triphosphate, 3H-labeled
2'-deoxycytidine 5'-triphosphate, magnesium salt
2'-deoxycytidine 5'-triphosphate, P''-(32)P-labeled
2'-deoxycytidine 5'-triphosphate, P-(32)P-labeled
dCTP
deoxycytidine triphosphate
2'-deoxycytidine 5'-triphosphate, 3H-labeled
2'-deoxycytidine 5'-triphosphate, magnesium salt
2'-deoxycytidine 5'-triphosphate, P''-(32)P-labeled
2'-deoxycytidine 5'-triphosphate, P-(32)P-labeled
dCTP
deoxycytidine triphosphate
Canonical SMILES
C1C(C(OC1N2C=CC(=NC2=O)N)COP(=O)([O-])OP(=O)([O-])OP(=O)([O-])[O-])O
Isomeric SMILES
C1[C@@H]([C@H](O[C@H]1N2C=CC(=NC2=O)N)COP(=O)([O-])OP(=O)([O-])OP(=O)([O-])[O-])O
Method of Synthesis or Extraction
The synthesis of 2'-Deoxycytidine-5'-triphosphate involves several steps, including the conversion of cytosine to cytidine, phosphorylation of cytidine to cytidine monophosphate (CMP), and subsequent phosphorylation of CMP to 2'-Deoxycytidine-5'-triphosphate. The efficiency and yield of each step depend on the specific conditions used, such as the choice of reagents, temperature, and pH. The overall yield of 2'-Deoxycytidine-5'-triphosphate synthesis is typically around 50-60%.
Environmental and safety considerations are important in the synthesis of 2'-Deoxycytidine-5'-triphosphate. The use of hazardous chemicals and solvents, such as acetonitrile and dimethylformamide, can pose risks to human health and the environment. Proper handling and disposal of these chemicals are necessary to minimize their impact.
Environmental and safety considerations are important in the synthesis of 2'-Deoxycytidine-5'-triphosphate. The use of hazardous chemicals and solvents, such as acetonitrile and dimethylformamide, can pose risks to human health and the environment. Proper handling and disposal of these chemicals are necessary to minimize their impact.
Chemical Structure and Biological Activity
The chemical structure of 2'-Deoxycytidine-5'-triphosphate consists of a cytosine base, a deoxyribose sugar, and three phosphate groups. It is structurally similar to other nucleotides, such as dATP, dGTP, and dTTP, which together make up the building blocks of DNA.
The biological activity of 2'-Deoxycytidine-5'-triphosphate is primarily related to its role in DNA synthesis and repair. It is incorporated into the growing DNA chain by DNA polymerase enzymes and serves as a template for the complementary base pairing of guanine. 2'-Deoxycytidine-5'-triphosphate also plays a role in regulating DNA replication and cell division.
The biological activity of 2'-Deoxycytidine-5'-triphosphate is primarily related to its role in DNA synthesis and repair. It is incorporated into the growing DNA chain by DNA polymerase enzymes and serves as a template for the complementary base pairing of guanine. 2'-Deoxycytidine-5'-triphosphate also plays a role in regulating DNA replication and cell division.
Biological Effects
The effects of 2'-Deoxycytidine-5'-triphosphate on cell function and signal transduction are complex and depend on the specific context. In general, 2'-Deoxycytidine-5'-triphosphate is essential for normal cell growth and division, and its depletion can lead to DNA damage and cell death.
Potential therapeutic and toxic effects of 2'-Deoxycytidine-5'-triphosphate are also being studied. Some studies have suggested that 2'-Deoxycytidine-5'-triphosphate may have anti-cancer properties, while others have shown that it can induce DNA damage and cell death in certain contexts.
Potential therapeutic and toxic effects of 2'-Deoxycytidine-5'-triphosphate are also being studied. Some studies have suggested that 2'-Deoxycytidine-5'-triphosphate may have anti-cancer properties, while others have shown that it can induce DNA damage and cell death in certain contexts.
Applications
In medical research, 2'-Deoxycytidine-5'-triphosphate is used in various applications, such as in drug development and clinical trials. It is also used in diagnostic assays, such as PCR, to amplify DNA sequences for detection.
In environmental research, 2'-Deoxycytidine-5'-triphosphate can be used to study the effects of pollutants on DNA damage and repair mechanisms. It can also be used in bioremediation processes to degrade pollutants in the environment.
In industrial research, 2'-Deoxycytidine-5'-triphosphate is used in manufacturing processes, such as the production of DNA sequencing reagents and diagnostic assays. It is also used to improve product quality and efficiency in various industries.
In environmental research, 2'-Deoxycytidine-5'-triphosphate can be used to study the effects of pollutants on DNA damage and repair mechanisms. It can also be used in bioremediation processes to degrade pollutants in the environment.
In industrial research, 2'-Deoxycytidine-5'-triphosphate is used in manufacturing processes, such as the production of DNA sequencing reagents and diagnostic assays. It is also used to improve product quality and efficiency in various industries.
Future Perspectives and Challenges
Current limitations in the use and study of 2'-Deoxycytidine-5'-triphosphate include its relatively low stability and susceptibility to degradation. Possible solutions and improvements include the development of more stable analogs and the optimization of synthesis methods.
Future trends and prospects in the application of 2'-Deoxycytidine-5'-triphosphate in scientific research include its continued use in drug development and diagnostic assays. It may also have potential applications in gene therapy and personalized medicine.
In conclusion, 2'-Deoxycytidine-5'-triphosphate is a crucial nucleotide that plays a vital role in DNA synthesis and repair. Its synthesis and use in various research applications require careful consideration of environmental and safety considerations. Further research is needed to fully understand its biological effects and potential therapeutic and toxic effects.
Future trends and prospects in the application of 2'-Deoxycytidine-5'-triphosphate in scientific research include its continued use in drug development and diagnostic assays. It may also have potential applications in gene therapy and personalized medicine.
In conclusion, 2'-Deoxycytidine-5'-triphosphate is a crucial nucleotide that plays a vital role in DNA synthesis and repair. Its synthesis and use in various research applications require careful consideration of environmental and safety considerations. Further research is needed to fully understand its biological effects and potential therapeutic and toxic effects.
Product FAQ
Q1: How Can I Obtain a Quote for a Product I'm Interested In?
- To receive a quotation, send us an inquiry about the desired product.
- The quote will cover pack size options, pricing, and availability details.
- If applicable, estimated lead times for custom synthesis or sourcing will be provided.
- Quotations are valid for 30 days, unless specified otherwise.
Q2: What Are the Payment Terms for Ordering Products?
- New customers generally require full prepayment.
- NET 30 payment terms can be arranged for customers with established credit.
- Contact our customer service to set up a credit account for NET 30 terms.
- We accept purchase orders (POs) from universities, research institutions, and government agencies.
Q3: Which Payment Methods Are Accepted?
- Preferred methods include bank transfers (ACH/wire) and credit cards.
- Request a proforma invoice for bank transfer details.
- For credit card payments, ask sales representatives for a secure payment link.
- Checks aren't accepted as prepayment, but they can be used for post-payment on NET 30 orders.
Q4: How Do I Place and Confirm an Order?
- Orders are confirmed upon receiving official order requests.
- Provide full prepayment or submit purchase orders for credit account customers.
- Send purchase orders to sales@thebiotek.com.
- A confirmation email with estimated shipping date follows processing.
Q5: What's the Shipping and Delivery Process Like?
- Our standard shipping partner is FedEx (Standard Overnight, 2Day, FedEx International Priority), unless otherwise agreed.
- You can use your FedEx account; specify this on the purchase order or inform customer service.
- Customers are responsible for customs duties and taxes on international shipments.
Q6: How Can I Get Assistance During the Ordering Process?
- Reach out to our customer service representatives at sales@thebiotek.com.
- For ongoing order updates or questions, continue using the same email.
- Remember, we're here to help! Feel free to contact us for any queries or further assistance.
Quick Inquiry
Note: Kindly utilize formal channels such as professional, corporate, academic emails, etc., for inquiries. The use of personal email for inquiries is not advised.
Category
Hot Products
D-ribose-L-cysteine
CAS No.: 232617-15-1
Sterubin
CAS No.: 51857-11-5
Troparil, (+)-
CAS No.: 74163-84-1
![N-Phenyl-d5-N'-[1-(2-phenylethyl)]-4-piperidine](/images/structure/BT-1439938.png)
N-Phenyl-d5-N'-[1-(2-phenylethyl)]-4-piperidine
CAS No.: 1189466-15-6
POE (15) tallow amine
CAS No.: 61791-26-2
5-Cholesten-3beta-25-diol-3-sulfate
CAS No.: 884905-07-1
info@thebiotek.com
256 N Vinedo Ave, Pasadena, California 91107
