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Orange G

Catalog Number: BT-264932

CAS Number: 1936-15-8

Molecular Formula: C16H10N2Na2O7S2

Molecular Weight: 452.4 g/mol

The product is for non-human research only. Not for therapeutic or veterinary use.

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Product Introduction

Description

Orange G is a synthetic dye that belongs to the azo dye family. It is commonly used in various industries, including food, textile, and paper. Orange G is also used in scientific research as a biological stain and a marker for protein electrophoresis.

Properties

CAS Number

1936-15-8

Product Name

Orange G

IUPAC Name

disodium;7-hydroxy-8-phenyldiazenylnaphthalene-1,3-disulfonate

Molecular Formula

C16H10N2Na2O7S2

Molecular Weight

452.4 g/mol

InChI

InChI=1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2

InChI Key

HSXUHWZMNJHFRV-QIKYXUGXSA-L

SMILES

C1=CC=C(C=C1)N=NC2=C(C=CC3=CC(=CC(=C32)S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+].[Na+]

Solubility

50 to 100 mg/mL at 74.3° F (NTP, 1992)
GENERALLY INSOL IN ORGANIC SOLVENTS, BUT SLIGHTLY SOL IN ETHANOL & CELLOSOLVE
Solubility in water = 80 mg/ml, in ethanol = 3 mg/ml, in methyl Cellosolve(monomethyl ether of ethylene glycol) = 40 mg/ml.

Synonyms

C.I. Orange G; C.I. 16230; NSC 10450; NSC-10450; NSC10450; Egacid Orange GG; Amacid Crystal Orange;

Canonical SMILES

C1=CC=C(C=C1)N=NC2=C(C=CC3=CC(=CC(=C32)S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+].[Na+]

Method of Synthesis or Extraction

Orange G is synthesized by coupling diazonium salt of sulfanilic acid with Naphthol AS. The reaction takes place in an alkaline medium, and the product is obtained as an orange powder. The yield of the reaction is around 80%, and the efficiency of the process is high. However, the synthesis of Orange G involves the use of toxic chemicals, such as sodium nitrite and hydrochloric acid, which can pose environmental and safety hazards.

Chemical Structure and Biological Activity

The chemical structure of Orange G consists of two aromatic rings linked by an azo group (-N=N-). The dye has a molecular weight of 452.4 g/mol and a melting point of 300°C. Orange G is a water-soluble dye that absorbs light in the visible region, with a maximum absorption at 480 nm. The biological activity of Orange G is attributed to its ability to bind to proteins and nucleic acids. It is commonly used as a biological stain for protein electrophoresis and as a marker for DNA sequencing.

Biological Effects

Orange G has been shown to affect cell function and signal transduction. It can induce oxidative stress and DNA damage in cells, leading to cell death. Orange G has also been shown to have potential therapeutic effects, such as anti-inflammatory and anti-cancer properties. However, Orange G can also have toxic effects, such as mutagenicity and carcinogenicity, which can pose health risks to humans and animals.

Applications

In medical research, Orange G is used as a biological stain for protein electrophoresis and as a marker for DNA sequencing. It has also been studied for its potential therapeutic effects, such as anti-inflammatory and anti-cancer properties. In environmental research, Orange G is used to study the effects of pollutants on ecosystems and to monitor pollution levels in water bodies. In industrial research, Orange G is used in manufacturing processes, such as dyeing textiles and paper, and improving product quality and efficiency. However, the use of Orange G in these applications can pose health and safety considerations.

Future Perspectives and Challenges

The current limitations in the use and study of Orange G include its potential health risks and environmental hazards. Possible solutions and improvements include the development of safer and more environmentally friendly methods of synthesis and extraction. Future trends and prospects in the application of Orange G in scientific research include the development of new therapeutic applications and the use of Orange G as a biosensor for detecting biomolecules. However, the challenges in the use and study of Orange G will require a multidisciplinary approach involving chemistry, biology, and environmental science.
Conclusion:
Orange G is a synthetic dye that has various applications in different industries and scientific research. The method of synthesis or extraction, chemical structure and biological activity, biological effects, applications, future perspectives, and challenges of Orange G have been discussed in this paper. The use of Orange G in different applications can pose health and safety considerations, and the development of safer and more environmentally friendly methods of synthesis and extraction is needed. The future trends and prospects in the application of Orange G in scientific research will require a multidisciplinary approach to address the challenges in its use and study.

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