Trypan blue
Materials Science
Trypan blue is a synthetic dye that is commonly used in medical, environmental, and industrial research. It is a dark blue dye that is soluble in water and has a molecular weight of 960.85 g/mol. Trypan blue is used to stain dead cells in biological samples, making it a valuable tool in cell viability assays. It is also used in environmental research to study the effects of pollutants on ecosystems and in industrial research to improve product quality and efficiency.
868.9 g/mol
868.9 g/mol
Formulation:
868.9 g/mol
Source:
Usage:
Trypan blue
The product is for non-human research only. Not for therapeutic or veterinary use.
Catalog Number: BT-308568
CAS Number: 72-57-1
Molecular Formula: C34H24N6Na4O14S4
Molecular Weight: 868.9 g/mol
Purity: ≥ 60%
Inventory: In Stock
| Size | SKU | Price | |
|---|---|---|---|
| 1g | bt-308568-1g | $304.31 | |
| 5g | bt-308568-5g | $752.31 | |
| 25g | bt-308568-25g | $2,020.15 | |
| 100g | bt-308568-100g | $5,739.54 |
| CAS Number | 72-57-1 |
|---|---|
| Product Name | Trypan blue |
| Molecular Formula | C34H24N6Na4O14S4 |
| Molecular Weight | 868.9 g/mol |
| Colorform | Bluish-grey powder Dark greenish-brown powder. Aqueous solution deep blue with violet tinge |
| Impurities | ... Substances identified in various samples /are/ 8-amino-2-[4'-(3,3' -dimethyl-biphenylazo)]-1-naphthol-3,6-disulfonic acid; and 8-amino-2-[4'-(3,3' -dimethyl-4-hydroxybiphenylazo)]-1-napthol-3,6-disulfonic acid; and 8-amino-2 -[4'-(3,3'-dimethyl-4-aminobiphenylazo)]-1-napthol-3,6-disulfonic acid ... and in addition ... can contain varying amt of its starting materials, including ortho-tolidine ... and inorganic salts. |
| InChI | InChI=1S/C34H28N6O14S4/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54)/p-4 |
| InChI Key | WMYVHJWZUUEZNE-ARWFNKCKSA-J |
| Isomeric SMILES | CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N/N=C\3/C(=O)C4=C(C=C3S(=O)(=O)[O-])C=C(C=C4N)S(=O)(=O)[O-])C)N/N=C\5/C(=O)C6=C(C=C5S(=O)(=O)[O-])C=C(C=C6N)S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+] |
| IUPAC Name | 5-amino-3-[[4-[4-[(8-amino-1-hydroxy-3,6-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-4-hydroxynaphthalene-2,7-disulfonate |
| Canonical SMILES | CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C(=CC4=C(C3=O)C(=CC(=C4)S(=O)(=O)[O-])N)S(=O)(=O)[O-])C)NN=C5C(=CC6=C(C5=O)C(=CC(=C6)S(=O)(=O)[O-])N)S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+] |
| Description | Trypan blue is a synthetic dye that is commonly used in medical, environmental, and industrial research. It is a dark blue dye that is soluble in water and has a molecular weight of 960.85 g/mol. Trypan blue is used to stain dead cells in biological samples, making it a valuable tool in cell viability assays. It is also used in environmental research to study the effects of pollutants on ecosystems and in industrial research to improve product quality and efficiency. |
| Method of Synthesis or Extraction | Trypan blue can be synthesized using a variety of methods, including the diazotization of sulfanilic acid and the coupling of the resulting diazonium salt with trypan blue. The efficiency and yield of each method vary depending on the specific conditions used. Environmental and safety considerations must also be taken into account when synthesizing trypan blue, as some of the chemicals used in the process can be hazardous. |
| Chemical Structure and Biological Activity | The chemical structure of trypan blue consists of a sulfonate group, a benzene ring, and a triphenylmethane group. The mechanism of action of trypan blue involves binding to nucleic acids in dead cells, causing them to become stained and easily distinguishable from live cells. Trypan blue has been shown to have low toxicity and is not known to have any significant biological activity beyond its use as a staining agent. |
| Biological Effects | Trypan blue has been shown to have minimal effects on cell function and signal transduction. It is not known to have any significant therapeutic effects, although it has been used in clinical trials to study the efficacy of certain drugs. Potential toxic effects of trypan blue are also minimal, although it should be used with caution in certain populations, such as pregnant women and individuals with kidney disease. |
| Applications | In medical research, trypan blue is commonly used to assess cell viability and to study the effects of drugs on cell death. It has also been used in clinical trials to study the efficacy of certain drugs. In environmental research, trypan blue is used to study the effects of pollutants on ecosystems and to assess the health of aquatic organisms. In industrial research, trypan blue is used to improve product quality and efficiency, particularly in the manufacturing of textiles and paper products. |
| Future Perspectives and Challenges | Current limitations in the use and study of trypan blue include its limited specificity for dead cells and its potential for false positives. Possible solutions and improvements include the development of more specific staining agents and the use of complementary assays to confirm cell viability. Future trends and prospects in the application of trypan blue in scientific research include its continued use as a valuable tool in cell viability assays and its potential for use in the development of new drugs and therapies. |
| Melting Point | greater than 572 °F (NTP, 1992) 300 °C |
| Other CAS Number | 2538-83-2 72-57-1 |
| Physical Description | Trypan blue is a bluish-gray to dark blue powder. (NTP, 1992) |
| Pictograms | Health Hazard |
| SMILES | CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O |
| Solubility | 1 to 10 mg/mL at 68° F (NTP, 1992) 20 mg/mL in methyl Cellosolve, and 0.6 mg/mL in ethanol Soluble in water In water, <0.1 mg/mL |
| Synonyms | 2,7-naphthalenedisulfonicacid,3,3’-((3,3’-dimethyl-4,4’-biphenylylene)bis(azo;2,7-naphthalenedisulfonicacid,3,3’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-;2,7-Naphthalenedisulfonicacid,3,3’-[[3,3’-dimethyl(1,1’-biphenyl)-4,4’-diyl]bis(azo)]bis(5-amino-4-h |
| PubChem Compound | Trypan blue |
| Last Modified | May 29 2023 |