Buy Trypan blue (BT-308568)

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Trypan blue

Catalog Number: BT-308568

CAS Number: 72-57-1

Molecular Formula: C34H24N6Na4O14S4

Molecular Weight: 868.9 g/mol

The product is for non-human research only. Not for therapeutic or veterinary use.

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Product Introduction

Description

Trypan blue is a synthetic dye that is commonly used in medical, environmental, and industrial research. It is a dark blue dye that is soluble in water and has a molecular weight of 960.85 g/mol. Trypan blue is used to stain dead cells in biological samples, making it a valuable tool in cell viability assays. It is also used in environmental research to study the effects of pollutants on ecosystems and in industrial research to improve product quality and efficiency.

Properties

CAS Number

72-57-1

Product Name

Trypan blue

IUPAC Name

5-amino-3-[[4-[4-[(8-amino-1-hydroxy-3,6-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-4-hydroxynaphthalene-2,7-disulfonate

Molecular Formula

C34H24N6Na4O14S4

Molecular Weight

868.9 g/mol

InChI

InChI=1S/C34H28N6O14S4/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54)/p-4

InChI Key

WMYVHJWZUUEZNE-ARWFNKCKSA-J

SMILES

CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O

Solubility

1 to 10 mg/mL at 68° F (NTP, 1992)
20 mg/mL in methyl Cellosolve, and 0.6 mg/mL in ethanol
Soluble in water
In water, <0.1 mg/mL

Synonyms

2,7-naphthalenedisulfonicacid,3,3’-((3,3’-dimethyl-4,4’-biphenylylene)bis(azo;2,7-naphthalenedisulfonicacid,3,3’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-;2,7-Naphthalenedisulfonicacid,3,3’-[[3,3’-dimethyl(1,1’-biphenyl)-4,4’-diyl]bis(azo)]bis(5-amino-4-h

Canonical SMILES

CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C(=CC4=C(C3=O)C(=CC(=C4)S(=O)(=O)[O-])N)S(=O)(=O)[O-])C)NN=C5C(=CC6=C(C5=O)C(=CC(=C6)S(=O)(=O)[O-])N)S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]

Isomeric SMILES

CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N/N=C\3/C(=O)C4=C(C=C3S(=O)(=O)[O-])C=C(C=C4N)S(=O)(=O)[O-])C)N/N=C\5/C(=O)C6=C(C=C5S(=O)(=O)[O-])C=C(C=C6N)S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]

Method of Synthesis or Extraction

Trypan blue can be synthesized using a variety of methods, including the diazotization of sulfanilic acid and the coupling of the resulting diazonium salt with trypan blue. The efficiency and yield of each method vary depending on the specific conditions used. Environmental and safety considerations must also be taken into account when synthesizing trypan blue, as some of the chemicals used in the process can be hazardous.

Chemical Structure and Biological Activity

The chemical structure of trypan blue consists of a sulfonate group, a benzene ring, and a triphenylmethane group. The mechanism of action of trypan blue involves binding to nucleic acids in dead cells, causing them to become stained and easily distinguishable from live cells. Trypan blue has been shown to have low toxicity and is not known to have any significant biological activity beyond its use as a staining agent.

Biological Effects

Trypan blue has been shown to have minimal effects on cell function and signal transduction. It is not known to have any significant therapeutic effects, although it has been used in clinical trials to study the efficacy of certain drugs. Potential toxic effects of trypan blue are also minimal, although it should be used with caution in certain populations, such as pregnant women and individuals with kidney disease.

Applications

In medical research, trypan blue is commonly used to assess cell viability and to study the effects of drugs on cell death. It has also been used in clinical trials to study the efficacy of certain drugs. In environmental research, trypan blue is used to study the effects of pollutants on ecosystems and to assess the health of aquatic organisms. In industrial research, trypan blue is used to improve product quality and efficiency, particularly in the manufacturing of textiles and paper products.

Future Perspectives and Challenges

Current limitations in the use and study of trypan blue include its limited specificity for dead cells and its potential for false positives. Possible solutions and improvements include the development of more specific staining agents and the use of complementary assays to confirm cell viability. Future trends and prospects in the application of trypan blue in scientific research include its continued use as a valuable tool in cell viability assays and its potential for use in the development of new drugs and therapies.

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