Recombinant T4 DNA Ligase
Enzymes
Formulation:
Source:
Escherichia Colilambda lysogen NM 989
Usage:
Recombinant T4 DNA Ligase
The product is for non-human research only. Not for therapeutic or veterinary use.
Catalog Number: BT-224146
Source: Escherichia Colilambda lysogen NM 989
Product Name | Recombinant T4 DNA Ligase |
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Bioactivity | One Weiss unit is equivalent to circa 67 cohesive-end ligation units.• T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.• Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50µM.• To dilute T4 DNA Ligase that will subsequently be stored at –20°C, 50% glycerol storage buffer should be used, to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used.Formulation |
Product Background | T4 DNA ligase is an Mg2+-dependent and ATP-dependent enzyme that seals DNA nicks in three steps: it covalently binds AMP, transadenylates the nick phosphate, and catalyses formation of the phosphodiester bond releasing AMP. |
Product Memo | T4 DNA T4 DNA Ligase Recombinant |
Product Type | Enzyme |
Source | Escherichia Colilambda lysogen NM 989 |
Synonym | DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.Description |
Last Modified | Aug 20 2021 |