SimpTM Taq DNA Polymerase
The product is for non-human research only. Not for therapeutic or veterinary use.
Catalog Number: BT-224148
Formulation: Taq DNA Polymerase solution in 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.
|Product Name||SimpTM Taq DNA Polymerase|
|Application||SimpTM Taq DNA Polymerase can be used for Routine PCR and Colony PCR|
|Components||SimpTM Taq DNA Polymerase, 10× SimpTaqTM Buffer, 2.5 mM dNTPs, 6×DNA Loading Buffer|
|Formulation||Taq DNA Polymerase solution in 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.|
|Description||SimpTM Taq DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. SimpTM Taq DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. SimpTM Taq DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE|
|Product Background||Taq DNA Polymerase(a) is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.|
|Product Memo||TaqDNA; Taq DNA Polymerase; Taq polymerase|
|Purity||Greater than 95.0% as determined by SDS-PAGE.|
|Storage||Store SimpTM TaqDNA Polymerase at -20°C for two years|
|Synonym||DNA polymerase I thermostable, EC 184.108.40.206, Taq polymerase 1.Taq DNA Polymerase|
|Usage||For Research Use Only|
|Last Modified||Aug 20 2021|