Recombinant Proteinase K Enzymes 29.3kD
$ $99 In stock
Formulation: 29.3kD
Source: Pichia pastoris
Usage: For Research Use Only

Recombinant Proteinase K

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog Number: BT-224149

Molecular Weight: 29.3kD

Formulation: White lyophilized powder or clear colorless 10 mM Tris-HCl (pH7.5) with 50% glycerol

Source: Pichia pastoris

Product Name Recombinant Proteinase K
Molecular Weight 29.3kD
Application Proteinase K is widely used in DNA/RNA preparations from tissue or cell cultures, and other industries.
Formulation White lyophilized powder or clear colorless 10 mM Tris-HCl (pH7.5) with 50% glycerol
Product Background In molecular biology Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium album proteinase K) is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).[5] Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 daltons (28.9 kDa).Proteinase K has two binding sites for Ca2+, which are located close to the active center, but are not directly involved in the catalytic mechanism. The residual activity is sufficient to digest proteins, which usually contaminate nucleic acid preparations. Therefore, the digestion with Proteinase K for the purification of nucleic acids is usually performed in the presence of EDTA (inhibition of calcium-dependent enzymes such as nucleases). Proteinase K is also stable over a wide pH range (4–12), with a pH optimum of pH 8.0.
Product Memo Proteinase K Recombinant
Product Type Enzyme
Source Pichia pastoris
Storage Store the Proteinase K at -20℃
Usage For Research Use Only
Last Modified Aug 20 2021