Recombinant Pfu DNA polymerase Enzymes
$ $99 In stock
Formulation:
Source: E. coli
Usage: For Research Use Only

Recombinant Pfu DNA polymerase

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog Number: BT-224154

Formulation: 50mM Tris-HCL (pH8.2), 1mM DTT, 0.1mM EDTA, 0.1% Tween 20, 0.1% Nonidet P-40 and 50% (v/v) glycerol.

Source: E. coli

Product Name Recombinant Pfu DNA polymerase
Application PCR, mutagenesis, ect
Formulation 50mM Tris-HCL (pH8.2), 1mM DTT, 0.1mM EDTA, 0.1% Tween 20, 0.1% Nonidet P-40 and 50% (v/v) glycerol.
Product Background Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus, where it functions to copy the organism's DNA during cell division. In the laboratory setting, Pfu is used to amplify DNA in the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a new strand of DNA during each extension step.Pfu DNA polymerase has superior thermostability and proofreading properties compared to Taq DNA polymerase. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity, meaning that as the DNA is assembled from the 5' end to 3' end, the exonuclease activity immediately removes nucleotides misincorporated at the 3' end of the growing DNA strand. Consequently, Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts.
Product Memo Pfu DNA polymerase Recombinant
Product Type Enzyme
Source E. coli
Storage Store the Proteinase K at -20℃
Usage For Research Use Only
Last Modified Aug 20 2021