Recombinant Pfu DNA polymerase
The product is for non-human research only. Not for therapeutic or veterinary use.
Catalog Number: BT-224154
Formulation: 50mM Tris-HCL (pH8.2), 1mM DTT, 0.1mM EDTA, 0.1% Tween 20, 0.1% Nonidet P-40 and 50% (v/v) glycerol.
Source: E. coli
|Product Name||Recombinant Pfu DNA polymerase|
|Application||PCR, mutagenesis, ect|
|Formulation||50mM Tris-HCL (pH8.2), 1mM DTT, 0.1mM EDTA, 0.1% Tween 20, 0.1% Nonidet P-40 and 50% (v/v) glycerol.|
|Product Background||Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus, where it functions to copy the organism's DNA during cell division. In the laboratory setting, Pfu is used to amplify DNA in the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a new strand of DNA during each extension step.Pfu DNA polymerase has superior thermostability and proofreading properties compared to Taq DNA polymerase. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity, meaning that as the DNA is assembled from the 5' end to 3' end, the exonuclease activity immediately removes nucleotides misincorporated at the 3' end of the growing DNA strand. Consequently, Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts.|
|Product Memo||Pfu DNA polymerase Recombinant|
|Storage||Store the Proteinase K at -20℃|
|Usage||For Research Use Only|
|Last Modified||Aug 20 2021|